A duplex droplet digital PCR assay for quantification of Alternaria spp. and Botrytis cinerea on sweet cherry at different growth stages

• Published in: Canadian journal of plant pathology Vol. 43; no. 5; pp. 734 - 748
• Main Authors: Larrabee, Melissa M., Voegel, Tanja M., Nelson, Louise M.
• Format: Journal Article
• Language: English
• Published: Philadelphia Taylor & Francis 03.09.2021; Taylor & Francis Ltd
• Subjects: Alternaria; Alternaria spp; Assaying; Botrytis cinerea; British Columbia; budbreak; cerisier de France; Cherries; color; detection limit; Disease; Disease control; Droplets; duplex droplet digital PCR; détection des agents pathogènes; Economic importance; Environmental conditions; flowering; Fruits; Fungicides; Growing season; Harvesting; intergenic DNA; internal transcribed spacers; pathogen detection; pathogen quantification; Pathogens; PCR numérique en gouttelettes utilisé en duplex; plant pathology; Polymerase chain reaction; Post-harvest decay; prediction; Prediction models; Prunus avium; quantification des agents pathogènes; Rot; Straw; sweet cherry; British Columbia Canada; Canada; British Columbia

Sweet cherries (Prunus avium) are an economically important crop in British Columbia, Canada. Cherries are harvested and distributed locally and overseas, where seemingly healthy fruit can succumb to postharvest diseases if disease conditions are met. Disease mitigation includes pre-harvest controls...