In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs’ cleavage by CRISPR/Cas13a system

• Published in: Antiviral research Vol. 178; p. 104794
• Main Authors: Chen, Yili, Jiang, Hongye, Wang, Ting, He, Dan, Tian, Rui, Cui, Zifeng, Tian, Xun, Gao, Qinglei, Ma, Xin, Yang, Jianrong, Wu, Jun, Tan, Songwei, Xu, Hongyan, Tang, Xiongzhi, Wang, Yan, Yu, Zhiying, Han, Hui, Das, Bhudev C., Severinov, Konstantin, Hitzeroth, Inga Isabel, Debata, Priya Ranjan, Xu, Wei, Fan, Weiwen, Jin, Zhuang, Cao, Chen, Yu, Miao, Xie, Weiling, Huang, Zhaoyue, Hu, Zheng, You, Zeshan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2020
• Subjects: Animals; Apoptosis; Cell Line, Tumor; Cell Proliferation; Cervical cancer; CRISPR-Cas Systems; CRISPR/Cas13a system; DNA Breaks, Double-Stranded; DNA-Binding Proteins - genetics; Down-Regulation; E6/E7; Female; Genetic Therapy; Growth inhibition; HeLa Cells; HPV 16/18; Human papillomavirus 16 - genetics; Human papillomavirus 18 - genetics; Humans; Mice; Mice, Inbred BALB C; Oncogene Proteins, Viral - genetics; Papillomavirus E7 Proteins - genetics; Papillomavirus Infections - virology; Repressor Proteins - genetics; Retinoblastoma Binding Proteins - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Viral - genetics; RNA, Viral - metabolism; Tumor suppression; Tumor Suppressor Protein p53 - genetics; Ubiquitin-Protein Ligases - genetics; Up-Regulation; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - virology; Xenograft Model Antitumor Assays; HPV 16/18; Tumor suppression; CRISPR/Cas13a system; Growth inhibition; E6/E7; Cervical cancer

Sustained infection of high-risk human papillomavirus (HR-HPVs), especially HPV16 and HPV18, is a major cause of cervical cancer. E6 and E7 oncoproteins, encoded by the HPV genome, are critical for transformation and maintenance of malignant phenotypes of cervical cancer. Here, we used an emerging p...