Adsorptive immobilization of rhodococcal cells in hydrophobized derivatives of wide-pore poly(acrylamide) cryogel

• Published in: Applied biochemistry and microbiology Vol. 47; no. 2; pp. 158 - 164
• Main Authors: Kuyukina, M. S, Ivshina, I. B, Rubtsova, E. V, Ivanov, R. V, Lozinsky, V. I
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.03.2011; SP MAIK Nauka/Interperiodica; Springer Nature B.V
• Subjects: Adsorption; Biochemistry; Biomedical and Life Sciences; Biophysics; Cellular biology; Hydrocarbons; Life Sciences; Medical Microbiology; Microbiology; Rhodococcus ruber; Immobilize Bacterial Cell; Allyl Glycidyl Ether; TMEDA; Apply Biochemistry
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683811020104

Adsorption of Rhodococcus ruber cells on columns with poly(acrylamide) cryogel (cryoPAAG) partially hydrophobized by different quantities (0.2, 1, and 5, mol %) of chemically grafted n-dodecane residues has been studied. The adsorption capacity (1.1 × 10⁹ cells/g) of gel carrier for rhodococcal cells and the optimal content (1 mol %) of hydrophobizing groups were determined. The respirometric method showed the high catalytic activity and functional stability of immobilized bacterial cells. Respiratory activity of immobilized rhodococci in the presence of a model mixture of oil hydrocarbons exceeded the respective parameter for free cells by 12-17%. Viability of rhodococcal cells adsorptionally fixed in hydrophobized cryoPAAG was maintained at a level of 93-95% after a half-year period of storage. The results may be used for development of immobilized biocatalyst for directed transformation of hydrocarbon compounds and biological purification of oil-polluted water.