Cadmium chloride elicitation of Abutilon indicum cell suspension cultures for enhanced stigmasterol production

Abutilon indicum (Malvaceae), a therapeutically valuable shrub can act as a continuous source of stigmasterol, accredited with pharmacological significance. In the present study, the content of stigmasterol when analyzed in both in vivo and in vitro plants was found to be 13.89 ± 1.43 and 20.50 ± 2....

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Published inPlant biosystems Vol. 156; no. 3; pp. 613 - 618
Main Authors Rao, Kiranmayee, Chodisetti, Bhuvaneswari, Gandi, Suryakala, Giri, Archana, Kavi Kishor, P. B.
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 07.07.2022
Taylor & Francis Ltd
Subjects
A. indicum
Abutilon indicum
Cadmium
Cadmium chloride
Callus
Cell suspensions
elicitation
humans
In vivo methods and tests
shrubs
stigmasterol
suspensions
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Abstract Abutilon indicum (Malvaceae), a therapeutically valuable shrub can act as a continuous source of stigmasterol, accredited with pharmacological significance. In the present study, the content of stigmasterol when analyzed in both in vivo and in vitro plants was found to be 13.89 ± 1.43 and 20.50 ± 2.34 µg/gFW, respectively. The callus obtained from the in vitro plants of A. indicum was found to contain 10.78 ± 0.19 µg/gFW of stigmasterol and was used for initiation of suspension cultures. In comparison to the calli, suspension cultures of A. indicum accumulated considerable amounts of stigmasterol (16.08 ± 1.92 µg/gFW) on the 12th day, i.e., end of log phase. The suspensions on further elicitation with Cadmium Chloride have shown a significant increase (2.59-fold) in the amount of stigmasterol compared to the initial calli, reaching 41.73 ± 3.77 µg/gFW. Thus, cell suspensions of A. indicum offer a unique advantage for large-scale production of stigmasterol under in vitro conditions, by retaining its natural essence and safety in human consumption.
AbstractList Abutilon indicum (Malvaceae), a therapeutically valuable shrub can act as a continuous source of stigmasterol, accredited with pharmacological significance. In the present study, the content of stigmasterol when analyzed in both in vivo and in vitro plants was found to be 13.89 ± 1.43 and 20.50 ± 2.34 µg/gFW, respectively. The callus obtained from the in vitro plants of A. indicum was found to contain 10.78 ± 0.19 µg/gFW of stigmasterol and was used for initiation of suspension cultures. In comparison to the calli, suspension cultures of A. indicum accumulated considerable amounts of stigmasterol (16.08 ± 1.92 µg/gFW) on the 12th day, i.e., end of log phase. The suspensions on further elicitation with Cadmium Chloride have shown a significant increase (2.59-fold) in the amount of stigmasterol compared to the initial calli, reaching 41.73 ± 3.77 µg/gFW. Thus, cell suspensions of A. indicum offer a unique advantage for large-scale production of stigmasterol under in vitro conditions, by retaining its natural essence and safety in human consumption.
Author Kavi Kishor, P. B.
Gandi, Suryakala
Rao, Kiranmayee
Giri, Archana
Chodisetti, Bhuvaneswari
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crossref_primary_10_3390_metabo14020127
crossref_primary_10_1016_j_plantsci_2025_112426
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SubjectTerms A. indicum
Abutilon indicum
Cadmium
Cadmium chloride
Callus
Cell suspensions
elicitation
humans
In vivo methods and tests
shrubs
stigmasterol
suspensions
Title Cadmium chloride elicitation of Abutilon indicum cell suspension cultures for enhanced stigmasterol production
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