In vitro regeneration, production, and storage of artificial seeds in Ceropegia barnesii, an endangered plant

• Published in: In vitro cellular & developmental biology. Plant Vol. 54; no. 5; pp. 553 - 563
• Main Authors: Ananthan, R., Mohanraj, Remya, Bai, V. Narmatha
• Format: Journal Article
• Language: English
• Published: New York Springer Science + Business Media, LLC (Springer) 01.10.2018; Springer US; Springer Nature B.V
• Subjects: Acids; Adenine; Alginic acid; Arbuscular mycorrhizas; artificial seeds; benzyladenine; Biomedical and Life Sciences; Cell Biology; Ceropegia; culture media; Developmental Biology; Embryonic growth stage; Embryos; Encapsulation; Endangered plants; Explants; Fabrication; Fungi; genetic engineering; Glomus aggregatum; habitats; Hardening; Herbal medicine; in vitro regeneration; in vitro studies; indole butyric acid; Indole-3-butyric acid; Inoculation; Life Sciences; Micropropagation; mycorrhizal fungi; Nodes; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Sciences; PLANT TISSUE CULTURE; plants (botany); Regeneration; Restoration; rooting; Seeds; Shoots; Sodium alginate; Somatic embryogenesis; Survival; survival rate; temperature; vesicular arbuscular mycorrhizae; Mumbai India; India; Encapsulated nodes; Direct regeneration; Cytokinins; Storage; Somatic embryogenesis; Auxins; Organogenesis

Approaches for in vitro regeneration and fabrication of synthetic seeds were formulated to support restoration in the wild and genetic manipulation of Ceropegia barnesii (categorized as endemic and endangered). MS medium augmented with 4 mg L−1 benzyl adenine was most advantageous for the production...