Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse
A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4‐methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and sub...
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| Published in | Journal of forensic sciences Vol. 64; no. 6; pp. 1906 - 1912 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
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United States
Wiley Subscription Services, Inc
01.11.2019
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| Abstract | A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4‐methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5 h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40–500 ng/mL. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100 μL of the sample was applied to the spot. The validation of the proposed method suggests a potential application in different scenarios in toxicology. |
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| AbstractList | Abstract
A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4‐methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5 h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40–500 ng/
mL
. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100 μL of the sample was applied to the spot. The validation of the proposed method suggests a potential application in different scenarios in toxicology. A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4‐methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5 h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40–500 ng/mL. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100 μL of the sample was applied to the spot. The validation of the proposed method suggests a potential application in different scenarios in toxicology. A liquid chromatography-mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4-methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5 h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40-500 ng/mL. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100 μL of the sample was applied to the spot. The validation of the proposed method suggests a potential application in different scenarios in toxicology. |
| Author | Santos, Maíra Kerpel dos Limberger, Renata Pereira Jacques, Ana Laura Bemvenuti |
| Author_xml | – sequence: 1 givenname: Ana Laura Bemvenuti surname: Jacques fullname: Jacques, Ana Laura Bemvenuti email: ana.jacques@ufrgs.br organization: Federal University of Rio Grande do Sul – sequence: 2 givenname: Maíra Kerpel dos surname: Santos fullname: Santos, Maíra Kerpel dos organization: Federal University of Rio Grande do Sul – sequence: 3 givenname: Renata Pereira surname: Limberger fullname: Limberger, Renata Pereira organization: Federal University of Rio Grande do Sul |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31206667$$D View this record in MEDLINE/PubMed |
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| Keywords | forensic toxicology investigative techniques analytic sample preparation methods analytical chemistry techniques dried oral fluid spot forensic science substance abuse detection |
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| Snippet | A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine,... A liquid chromatography-mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine,... Abstract A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of... |
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| SubjectTerms | Acetonitrile analytic sample preparation methods analytical chemistry techniques Chromatography, Liquid Desiccation dried oral fluid spot Drug abuse forensic science forensic toxicology Forensic Toxicology - methods Humans Illicit Drugs - analysis investigative techniques Ions Linearity Liquid chromatography Mass Spectrometry Narcotics Saliva - chemistry Selectivity Stability analysis substance abuse detection Substance Abuse Detection - methods Substance-Related Disorders - diagnosis Toxicology |
| Title | Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2F1556-4029.14112 https://www.ncbi.nlm.nih.gov/pubmed/31206667 https://www.proquest.com/docview/2310958017/abstract/ |
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