Optimization of 1,2,4‐butanetriol production from xylose in Saccharomyces cerevisiae by metabolic engineering of NADH/NADPH balance

• Published in: Biotechnology and bioengineering Vol. 118; no. 1; pp. 175 - 185
• Main Authors: Yukawa, Takahiro, Bamba, Takahiro, Guirimand, Gregory, Matsuda, Mami, Hasunuma, Tomohisa, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.01.2021
• Subjects: 1,2,4‐butanetriol; Alcohol dehydrogenase; Chemical reactions; coenzyme balance; Dehydration; Dehydrogenase; Dehydrogenases; Genetic engineering; Genomes; Glucose; Kinases; Low concentrations; Metabolic engineering; Metabolism; Mitochondria; NADH; NADH kinase; Nicotinamide adenine dinucleotide; Optimization; Saccharomyces cerevisiae; Xylonate dehydratase; Xylose; Yeast; 1,2,4-butanetriol; coenzyme balance; Saccharomyces cerevisiae; xylose

1,2,4‐Butanetriol (BT) is used as a precursor for the synthesis of various pharmaceuticals and the energetic plasticizer 1,2,4‐butanetriol trinitrate. In Saccharomyces cerevisiae, BT is biosynthesized from xylose via heterologous four enzymatic reactions catalyzed by xylose dehydrogenase, xylonate d...