Tissue refractometry using Hilbert phase microscopy

• Published in: Optics letters Vol. 32; no. 24; p. 3522
• Main Authors: Lue, Niyom, Bewersdorf, Joerg, Lessard, Mark D, Badizadegan, Kamran, Dasari, Ramachandra R, Feld, Michael S, Popescu, Gabriel
• Format: Journal Article
• Language: English
• Published: United States 15.12.2007
• Subjects: Animals; Brain - pathology; Brain Mapping; Disease Models, Animal; Image Interpretation, Computer-Assisted - methods; Liver - pathology; Liver Diseases - pathology; Mice; Mice, Inbred C57BL; Microscopy, Phase-Contrast - instrumentation; Microscopy, Phase-Contrast - methods; Models, Statistical; Refractometry - instrumentation; Refractometry - methods; Spleen - pathology; Tissue Distribution

We present, for the first time to our knowledge, quantitative phase images associated with unstained 5 mum thick tissue slices of mouse brain, spleen, and liver. The refractive properties of the tissue are retrieved in terms of the average refractive index and its spatial variation. We find that the...