Preparation and identification of novel inhibitory angiotensin-I-converting enzyme peptides from tilapia skin gelatin hydrolysates: inhibition kinetics and molecular docking

• Published in: Food & function Vol. 9; no. 1; pp. 5251 - 5259
• Main Authors: Ling, Yuan, Liping, Sun, Yongliang, Zhuang
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 17.10.2018
• Subjects: active sites; Amino acids; Angiotensin; Angiotensin-Converting Enzyme Inhibitors - chemistry; Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Angiotensin-Converting Enzyme Inhibitors - metabolism; Animals; Biological Transport; Caco-2 Cells; chromatography; computer simulation; Conversion; Digestive system; Enzymes; Fish Proteins - chemistry; Fish Proteins - isolation & purification; Fish Proteins - metabolism; functional foods; Functional foods & nutraceuticals; gastrointestinal system; Gastrointestinal tract; Gelatin; Gelatin - chemistry; human cell lines; Humans; Hydrogen Bonding; Hydrogen bonds; Hydrolysates; Hydrolysis; Hypertension; in vitro digestion; ingredients; inhibitory concentration 50; Kinetics; Molecular docking; Molecular Docking Simulation; Peptide Mapping; Peptides; Peptides - chemistry; Peptides - isolation & purification; Peptides - metabolism; Peptidyl-Dipeptidase A - chemistry; Peptidyl-Dipeptidase A - metabolism; Protein Hydrolysates - chemistry; Skin; Skin - chemistry; Skin preparations; Tilapia; Transport

Tilapia skin gelatin was hydrolyzed by successive simulated gastrointestinal digestion, and the hydrolysates were further separated by transport across a Caco-2 cell monolayer. Angiotensin-I-converting enzyme inhibitory (ACEI) peptides were separated by successive chromatographic steps from the tran...