Proteoglycans isolated from dissociative extracts of differently aged human articular cartilage: characterization of naturally occurring hyaluronan-binding fragments of aggrecan

• Published in: Biochemical journal Vol. 304 ( Pt 3); no. 3; pp. 887 - 894
• Main Authors: Vilim, V, Fosang, A J
• Format: Journal Article
• Language: English
• Published: England 15.12.1994
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Aggrecans; Aging - metabolism; Cartilage, Articular - chemistry; Cartilage, Articular - metabolism; Catalysis; Centrifugation, Density Gradient; Electrophoresis, Polyacrylamide Gel; Endopeptidases - metabolism; Extracellular Matrix Proteins; Humans; Hyaluronic Acid - analysis; Hyaluronic Acid - metabolism; Lectins, C-Type; Proteoglycans - analysis; Proteoglycans - isolation & purification; Proteoglycans - metabolism

Proteoglycans extracted with 4 M guanidinium chloride from young (mean 20 years) or old (mean 79 years) macroscopically normal human articular cartilage were separated by density gradient centrifugation and Q-Sepharose chromatography and characterized by gradient gel SDS/PAGE and immunodetection bef...