Sequence Analysis and Preliminary X-ray Crystallographic Analysis of an Acetylesterase (LgEstI) from Lactococcus garvieae

• Published in: Crystals (Basel) Vol. 12; no. 1; p. 46
• Main Authors: Do, Hackwon, Wang, Ying, Lee, Chang Woo, Yoo, Wanki, Jeon, Sangeun, Hwang, Jisub, Lee, Min Ju, Kim, Kyeong Kyu, Kim, Han-Woo, Lee, Jun Hyuck, Kim, T. Doohun
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.01.2022
• Subjects: Bacteria; Calcium acetate; Cloning; Crystal structure; Crystallization; Crystallography; Data collection; Drug resistance; E coli; Enzymes; esterase; Glycerol; Lactococcus garvieae; Molecular structure; Molecular weight; Proteins; Substrates; Vectors (Biology); X-ray crystallography; United States > US

A gene encoding LgEstI was cloned from a bacterial fish pathogen, Lactococcus garvieae. Sequence and bioinformatic analysis revealed that LgEstI is close to the acetyl esterase family and had maximum similarity to a hydrolase (UniProt: Q5UQ83) from Acanthamoeba polyphaga mimivirus (APMV). Here, we p...