Determination of Catechins and Purine Alkaloids in Tea by High Performance Liquid Chromatography

• Published in: Analytical letters Vol. 45; no. 17; pp. 2530 - 2537
• Main Authors: Tan, Heping, Xu, Wenping, Zhao, Aiping, Zhou, Lili, Liu, Mingdong, Tan, Fuyuan, Zou, Yan, Wang, Yuejie
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 15.11.2012; Taylor & Francis
• Subjects: 8-chlorotheophylline; Alkaloids; Analysis; Analytical chemistry; Catechin; Catechins; Chemistry; Chromatographic methods and physical methods associated with chromatography; Elution; Exact sciences and technology; High performance liquid chromatography; Internal standard; Liquid chromatography; Other chromatographic methods; Purine alkaloid; Purines; Tea; Elution; Gradient; Temperature; Catechin; Gallic acid; Internal standard; HPLC chromatography; 8-chlorotheophylline; Method; Mobile phase; Wavelength; Chemical enrichment; Purine alkaloid; Alkaloid; Accuracy; Adsorption; Analysis; Acetonitrile; Simultaneous measurement; Aqueous solution; Acetic acid; Detection; Variation coefficient

With a novel internal standard, 8-chlorotheophylline, a high-performance liquid chromatography method was successfully developed for simultaneous determination of six catechins, three purine alkaloids, and gallic acid in green tea, oolong tea, and black tea. The analysis was carried out on a C18 col...