Chondrogenic Priming Adipose-Mesenchymal Stem Cells for Cartilage Tissue Regeneration
ABSTRACT Purpose Chondrocytes lose their ability to produce cartilaginous matrix during multiplication in culture through repeated passages, resulting in inferior tissue phenotype. To overcome the limited amount of primary chondrocytes, we aimed to determine the optimal culture condition for in vitr...
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Published in | Pharmaceutical research Vol. 28; no. 6; pp. 1395 - 1405 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Boston
Springer US
01.06.2011
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | ABSTRACT
Purpose
Chondrocytes lose their ability to produce cartilaginous matrix during multiplication in culture through repeated passages, resulting in inferior tissue phenotype. To overcome the limited amount of primary chondrocytes, we aimed to determine the optimal culture condition for
in vitro
/
in vivo
cartilage regeneration using human adipose-derived mesenchymal stem cells (AMSCs).
Methods
To evaluate the effects exerted by the chondrocytic culture condition on AMSC, we utilized chondrocyte conditioned medium (CM) and/or co-culture methods to prime and differentiate AMSCs. We evaluated ultimate
in vivo
engineered cartilage with primed AMSCs with that of chondrocytes. To examine the link between conditioned factors and proliferation/differentiation, cell cycle progression of AMSCs were examined using 5-ethynyl-2′-deoxyuridine (EdU), and gene expression was monitored.
Results
We report that AMSCs can be stimulated to become chondrogenic cells when expanded with chondrocyte CM. Polymeric scaffolds co-seeded with CM- expanded AMSCs and primary chondrocytes resulted in
in vivo
cartilaginous tissues with similar biochemical content to constructs seeded with chondrocytes alone.
Conclusion
These results indicate that chondrocyte CM consists of suitable morphogenetic factors that induce the chondrogenic priming of AMSCs for cartilage tissue engineering. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0724-8741 1573-904X |
DOI: | 10.1007/s11095-011-0445-2 |