Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle

• Published in: BioTechniques Vol. 76; no. 9; pp. 415 - 423
• Main Authors: Lu, Huiying, Wang, Xiaofeng
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 2024
• Subjects: AuNPs; Biosensing Techniques - methods; DNA, Catalytic - chemistry; DNA, Catalytic - metabolism; Exodeoxyribonucleases - chemistry; Exodeoxyribonucleases - metabolism; exonuclease-III; glucose; Glucose - analysis; Glucose - metabolism; glucose oxidase; Glucose Oxidase - chemistry; Glucose Oxidase - metabolism; Gold - chemistry; Humans; Limit of Detection; Metal Nanoparticles - chemistry; pistol-like DNAzyme (PLDz); glucose; exonuclease-III; pistol-like DNAzyme (PLDz); AuNPs; glucose oxidase
• DOI: 10.1080/07366205.2024.2381403
• ISSN: 0736-6205; 1940-9818

Detecting glucose accurately and sensitively from clinical samples like tears and saliva is still difficult. We have created a sensor that can detect glucose with high sensitivity and accuracy by combining the use of glucose oxidase (GOx) to catalyze glucose, a pistol-like DNAzyme (PLDz) to transform the signal, gold nanoparticles (AuNPs) to enhance the optical properties and the exonuclease-III (Exo-III) to amplify the signal. As a result, the proposed method exhibits a low detection limit of 7.5 pM and a wide detection range covering seven orders of magnitude. The suggested dual-mode strategy provides a sensitive, precise and specific detection method for glucose. Another advantage is that the dual-mode technique significantly improves the precision and consistency of the measurements, demonstrating its immense potential for use in biomedical research and clinical diagnostics.