A Strategy for Detecting Absorbed Bioactive Compounds for Quality Control in the Water Extract of Rhubarb by Ultra Performance Liquid Chromatography with Photodiode Array Detector

Objective： To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Methods： Anthraquinones in water extract of rhubarb （WER）, in cerebrospinal fluid （CSF） of patients with traumatic brain injury （TBI） and...

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Published inChinese journal of integrative medicine Vol. 18; no. 9; pp. 690 - 698
Main Author 王杨 黄熙 梁清华 范荣 秦锋 呙勇 闫奎坡 刘维 罗杰坤 李云辉 毛喜莲 刘昭前 周宏灏
Format Journal Article
LanguageEnglish
Published Heidelberg Chinese Association of Traditional and Western Medicine 01.09.2012
Subjects
Absorption - drug effects
Animals
Anthraquinones - cerebrospinal fluid
Anthraquinones - chemistry
Biological Products - analysis
Biological Products - cerebrospinal fluid
Biological Products - chemistry
Brain Injuries - drug therapy
Brain Injuries - pathology
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
Emodin - administration & dosage
Emodin - analogs & derivatives
Emodin - pharmacology
Emodin - therapeutic use
Humans
Limit of Detection
Linear Models
Male
Medicine
Medicine & Public Health
Original Article
Plant Extracts - chemistry
Quality Control
Rats
Rats, Sprague-Dawley
Reference Standards
Reproducibility of Results
Rheum - chemistry
Water - chemistry
人体吸收
光电二极管阵列检测器
水提取物
生物活性化合物
芦荟大黄素
质量控制
超氧化物歧化酶活性
超高效液相色谱
craniocerebral injury
rhubarb
absorbed bioactive compounds
anthraquinones
quality control
ultra performance liquid chromatography
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Summary:Objective： To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Methods： Anthraquinones in water extract of rhubarb （WER）, in cerebrospinal fluid （CSF） of patients with traumatic brain injury （TBI） and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector （UPLC-PDA） method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase （SOD） activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER. Results： Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly. Conclusions： Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.
Bibliography:quality control;absorbed bioactive compounds;rhubarb;ultra performance liquid chromatography;craniocerebral injury;anthraquinones
WANG Yang,HUANG Xi,LIANG Qing-hua,FAN Rong,QIN Feng,GUO Yong,YAN Kui-po,LIU Wei,LUO Jie-kun,LI Yun-hui,MAO Xi-lian,LIU Zhao-qian,and ZHOU Hong-hao 1, Institute of Integrated Traditional Chinese and Western Medicine, and the National Key Clinical Specialist Vocational School of Traditional Chinese Medicine Encephalopathy, Xiangya Hospital, Central South University, Changsha （410008）, China; 2. Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha （410008）, China; 3. Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha （410008）, China; 4. Institute of Clinical Pharmacology, Central South University, Changsha （410008）, China
11-4928/R
Objective： To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Methods： Anthraquinones in water extract of rhubarb （WER）, in cerebrospinal fluid （CSF） of patients with traumatic brain injury （TBI） and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector （UPLC-PDA） method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase （SOD） activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER. Results： Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly. Conclusions： Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.
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SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1672-0415
1993-0402
1993-0402
DOI:10.1007/s11655-012-1053-7