Establishment of human cell lines lacking mitochondrial DNA

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 554; p. 383
• Main Authors: Hashiguchi, Kazunari, Zhang-Akiyama, Qiu-Mei
• Format: Journal Article
• Language: English
• Published: United States 2009
• Subjects: Cell Culture Techniques - methods; Cells, Cultured; DNA, Mitochondrial - genetics; Ethidium - pharmacology; Humans; Indicators and Reagents - pharmacology; Mitochondria - drug effects; Mitochondria - metabolism; Polymerase Chain Reaction - methods

Mitochondria have their own genome, and mitochondrial DNA (mtDNA) encodes 2 ribosomal RNAs, 22 transfer RNAs, and 13 polypeptides that function in oxidative phosphorylation (OXPHOS). mtDNA mutations lead to dysfunction of OXPHOS, resulting in cell death and/or compromised cellular activity. Cell lin...