Single-Cell RNA Sequencing Reveals Expanded Clones of Islet Antigen-Reactive CD4+ T Cells in Peripheral Blood of Subjects with Type 1 Diabetes

• Published in: The Journal of immunology (1950) Vol. 199; no. 1; pp. 323 - 335
• Main Authors: Cerosaletti, Karen, Barahmand-pour-Whitman, Fariba, Yang, Junbao, DeBerg, Hannah A, Dufort, Matthew J, Murray, Sara A, Israelsson, Elisabeth, Speake, Cate, Gersuk, Vivian H, Eddy, James A, Reijonen, Helena, Greenbaum, Carla J, Kwok, William W, Wambre, Erik, Prlic, Martin, Gottardo, Raphael, Nepom, Gerald T, Linsley, Peter S
• Format: Journal Article
• Language: English
• Published: United States American Association of Immunologists 01.07.2017
• Subjects: Adult; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; Cell activation; Clone Cells; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 1 - immunology; Female; Gene Expression Profiling; Humans; Immunologic Memory; Immunological memory; Islets of Langerhans - immunology; Lymphocyte Activation; Lymphocytes; Lymphocytes T; Male; Memory cells; Peptides - immunology; Peripheral blood; Phenotype; Receptors, Antigen, T-Cell, alpha-beta - immunology; Ribonucleic acid; RNA; Sequence Analysis, RNA; Single-Cell Analysis; T cell receptors; Transcription

The significance of islet Ag-reactive T cells found in peripheral blood of type 1 diabetes (T1D) subjects is unclear, partly because similar cells are also found in healthy control (HC) subjects. We hypothesized that key disease-associated cells would show evidence of prior Ag exposure, inferred fro...