Assessment of a Micropatterned Hepatocyte Coculture System to Generate Major Human Excretory and Circulating Drug Metabolites

• Published in: Drug metabolism and disposition Vol. 38; no. 10; pp. 1900 - 1905
• Main Authors: Wang, Wendy WeiWei, Khetani, Salman R., Krzyzewski, Stacy, Duignan, David B., Obach, R. Scott
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.10.2010; American Society for Pharmacology and Experimental Therapeutics
• Subjects: Biological and medical sciences; Cells, Cultured; Chromatography, High Pressure Liquid; Coculture Techniques - methods; Cryopreservation; Hepatocytes - metabolism; Humans; Medical sciences; Metabolic Detoxication, Phase I; Metabolic Detoxication, Phase II; Models, Biological; Molecular Structure; Pharmaceutical Preparations - chemistry; Pharmaceutical Preparations - metabolism; Pharmacology. Drug treatments; Predictive Value of Tests; Tandem Mass Spectrometry; CP-122721; HCM; CP-533536; ADME; CP-547632; CP-690550; CJ-13610; HPLC; Human; Drug; Evaluation; Mixed cell culture; Hepatocyte; Digestive system; Metabolite; Liver; In vitro

Metabolism is one of the important determinants of the overall disposition of drugs, and the profile of metabolites can have an impact on efficacy and safety. Predicting which drug metabolites will be quantitatively predominant in humans has become increasingly important in the research and developm...