Identification of naturally processed T cell epitopes from glutamic acid decarboxylase presented in the context of HLA-DR alleles by T lymphocytes of recent onset IDDM patients

• Published in: The Journal of clinical investigation Vol. 99; no. 10; pp. 2405 - 2415
• Main Authors: Endl, J, Otto, H, Jung, G, Dreisbusch, B, Donie, F, Stahl, P, Elbracht, R, Schmitz, G, Meinl, E, Hummel, M, Ziegler, A G, Wank, R, Schendel, D J
• Format: Journal Article
• Language: English
• Published: United States 15.05.1997
• Subjects: Alleles; Amino Acid Sequence; Antibodies, Monoclonal; Antigen-Presenting Cells - immunology; Cell Line; Cells, Cultured; Cerebellum - enzymology; Diabetes Mellitus, Type 1 - immunology; Epitopes - analysis; Epitopes - chemistry; Glutamate Decarboxylase - chemistry; Glutamate Decarboxylase - immunology; Haplotypes; HLA-DR Antigens - genetics; Humans; Interferon-gamma - biosynthesis; Interleukin-2 - biosynthesis; Interleukin-4 - biosynthesis; Interleukin-6 - biosynthesis; Lymphocyte Activation; Male; Molecular Sequence Data; T-Lymphocytes - immunology

Glutamic acid decarboxylase (GAD) has been defined as a major target antigen in insulin-dependent diabetes mellitus (IDDM). To identify the molecular ligands triggering a T cell response to GAD, a panel of human GAD65-specific T lymphocyte lines was generated from peripheral blood of three recent on...