Identification of Lithocholic Acid and Measurement of Other Bile Acids in Serum of Healthy Humans

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 19; no. 2; pp. 248 - 252
• Main Authors: Pellizzari, E. D, O’Neil, F. S, Farmer, R. W, Fabre, L. F., Jr
• Format: Journal Article
• Language: English
• Published: England Am Assoc Clin Chem 01.02.1973
• Subjects: Amidohydrolases; Bile Acids and Salts - blood; Carbon Isotopes; Chenodeoxycholic Acid - blood; Cholic Acids - blood; Chromatography, Gas; Chromatography, Ion Exchange; Clostridium perfringens - enzymology; Deoxycholic Acid - blood; Fasting; Glycocholic Acid - blood; Humans; Lithocholic Acid - blood; Mass Spectrometry; Methods

Combined gas—liquid chromatography mass-spectroscopy was used to identify lithocholic acid and confirm the presence of other bile acids in serum of a healthy fasting subject. GLC was used to measure deoxycholic (DCA), chenodeoxycholic (CDCA), and cholic (CA) acids in sera. Before analysis, serum bil...