GPI-anchored complement regulatory proteins in seminal plasma. An analysis of their physical condition and the mechanisms of their binding to exogenous cells

• Published in: The Journal of clinical investigation Vol. 97; no. 7; pp. 1675 - 1686
• Main Authors: Rooney, I A, Heuser, J E, Atkinson, J P
• Format: Journal Article
• Language: English
• Published: United States 01.04.1996
• Subjects: Animals; Antigens, CD - metabolism; Antigens, Neoplasm; CD52 Antigen; CD55 Antigens - metabolism; CD59 Antigens - metabolism; CHO Cells; Complement System Proteins - metabolism; Cricetinae; Erythrocytes - immunology; Erythrocytes - metabolism; Glycoproteins; Glycosylphosphatidylinositols - metabolism; Humans; In Vitro Techniques; Macrophages, Peritoneal - immunology; Macrophages, Peritoneal - metabolism; Male; Membrane Cofactor Protein; Membrane Glycoproteins - metabolism; Mice; Microscopy, Electron; Organelles - immunology; Organelles - metabolism; Organelles - ultrastructure; Protein Binding; Rats; Semen - cytology; Semen - immunology; Semen - metabolism

We analyzed and compared the properties of three glycosylphosphatidylinositol (GPI)-anchored proteins. CD59, CD55 (both C regulators), and CDw52, and of the transmembrane C regulator CD46 in seminal plasma (SP). We demonstrated previously that anchor-intact SP CD59 is present on the membranes of ves...