Targeted On‐line SPE‐LC‐MS/MS Assay for the Quantitation of 12 Apolipoproteins from Human Blood

• Published in: Proteomics (Weinheim) Vol. 18; no. 3-4
• Main Authors: Dittrich, Julia, Adam, Melanie, Maas, Hilke, Hecht, Max, Reinicke, Madlen, Ruhaak, L. Renee, Cobbaert, Christa, Engel, Christoph, Wirkner, Kerstin, Löffler, Markus, Thiery, Joachim, Ceglarek, Uta
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.02.2018
• Subjects: Aged; Apolipoproteins; Apolipoproteins - blood; Assaying; Biological properties; Biological samples; Cholesterol; Chromatography, Liquid - methods; Coagulants; Confounding (Statistics); confounding variables; Female; High-Throughput Screening Assays - methods; Humans; Immunoassays; Lipid metabolism; Male; Metabolism; Middle Aged; Online Systems; preanalytics; Pretreatment; Proteomics; Proteomics - methods; Quantitation; quantitative proteomics; Sampling; Solid Phase Extraction - methods; Tandem Mass Spectrometry - methods; targeted proteomics; Triglycerides; Trypsin; targeted proteomics; apolipoproteins; preanalytics; confounding variables; quantitative proteomics
• ISSN: 1615-9853; 1615-9861; 1615-9861
• DOI: 10.1002/pmic.201700279

Laborious sample pretreatment of biological samples represents the most limiting factor for the translation of targeted proteomics assays from research to clinical routine. An optimized method for the simultaneous quantitation of 12 major apolipoproteins (apos) combining on‐line SPE and fast LC‐MS/M...