VvpM Induces Human Cell Death via Multifarious Modes Including Necroptosis and Autophagy

• Published in: Journal of microbiology and biotechnology Vol. 25; no. 2; pp. 302 - 306
• Main Authors: Lee, Mi-Ae, Kim, Jeong-A, Shin, Mee-Young, Lee, Jeong K., Park, Soon-Jung, Lee, Kyu-Ho
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 01.02.2015
• Subjects: Apoptosis; Autophagy; Caspase 3 - metabolism; Caspases - metabolism; Cell Death; Cell Line, Tumor; Cell Survival; Cytochromes c - metabolism; Humans; Imidazoles - pharmacology; Indoles - pharmacology; Metalloproteases - metabolism; Necrosis; Vibrio vulnificus - enzymology; Vibrio vulnificus - metabolism; Vibrio vulnificus - pathogenicity; 생물학
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1501.01007

VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus, induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). The percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1, indicating that VvpM-mediated cell death occurs through necroptosis. The appearance of autophagic vesicles and lipidated form of light-chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, the multifarious action of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.