Total integrated centrifugal genetic analyzer for point-of-care Covid-19 testing with automatic and high-throughput capability

• Published in: Sensors and actuators. B, Chemical Vol. 353; p. 131088
• Main Authors: Nguyen, Hau Van, Phan, Vu Minh, Seo, Tae Seok
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15.02.2022; Elsevier Science Ltd
• Subjects: Capillary pressure; Centrifugal microfluidics; Chambers; Coronaviruses; COVID-19; COVID-19 diagnostic tests; Fluorescence; High-throughput; Loop-mediated isothermal amplification (LAMP); Point-of-care testing; Portability; Ribonucleic acid; RNA; Workstations; High-throughput; Point-of-care testing; Centrifugal microfluidics; Covid-19; Loop-mediated isothermal amplification (LAMP)

We propose a prototype portable genetic analyzer, which performs the entire processes including RNA purification, reverse transcription multiplex loop-mediated isothermal amplification (RT-LAMP), and real-time fluorescence detection. Ten units of integrated functional entity were incorporated on a c...