Immobilization of xylanase on modified grafted alginate polyethyleneimine bead based on impact of sodium cation effect

• Published in: International journal of biological macromolecules Vol. 140; pp. 1284 - 1295
• Main Authors: Mostafa, Faten A., El Aty, Abeer A.A., Hassan, Mohamed E., Awad, Ghada E.A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2019
• Subjects: Alginates - chemistry; Aspergillus flavus - enzymology; Biocatalysis; Cations; Covalent immobilization; Effect of sodium cation; Endo-1,4-beta Xylanases - metabolism; Enzyme Stability; Enzymes, Immobilized - metabolism; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Microspheres; Polyethyleneimine - chemistry; Sodium - chemistry; Spectrometry, X-Ray Emission; Spectroscopy, Fourier Transform Infrared; Temperature; Xylanase; Xylans - metabolism; Xylanase; Effect of sodium cation; Covalent immobilization

Alginate- polyethyleneimine gel beads modified by using 0.3 M Na+ were used for covalent immobilization of Aspergillus flavus xylanase. SEM images showed distorted structure with addition of Na+ that impaired the egg-box structure formation offered much covalent binding with xylanase. Immobilization...