Repeat treatment of organotypic airway cultures with ethyl methanesulfonate causes accumulation of somatic cell mutations without expansion of bronchial-carcinoma-specific cancer driver mutations

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis Vol. 897; p. 503786
• Main Authors: Wang, Yiying, Le, Yuan, Harris, Kelly L., Chen, Ying, Li, Xilin, Faske, Jennifer, Wynne, Rebecca A., Mittelstaedt, Roberta A., Cao, Xuefei, Miranda-Colon, Jaime, Elkins, Lana, Muskhelishvili, Levan, Davis, Kelly, Mei, Nan, Sun, Wei, Robison, Timothy W., Heflich, Robert H., Parsons, Barbara L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2024
• Subjects: Bronchi - cytology; Bronchi - drug effects; DNA damage; DNA Damage - drug effects; Error-corrected next generation sequencing (ecNGS); Ethyl methanesulfonate (EMS); Ethyl Methanesulfonate - pharmacology; Ethyl Methanesulfonate - toxicity; Human in vitro organotypic air-liquid-interface (ALI) airway tissue model; Humans; Mutagenesis; Mutagenesis - drug effects; Mutagens - toxicity; Mutation - drug effects; New approach methods (NAMs); New approach methods (NAMs); Mutagenesis; Human in vitro organotypic air-liquid-interface (ALI) airway tissue model; DNA damage; Ethyl methanesulfonate (EMS); Error-corrected next generation sequencing (ecNGS)

The human in vitro organotypic air-liquid-interface (ALI) airway tissue model is structurally and functionally similar to the human large airway epithelium and, as a result, is being used increasingly for studying the toxicity of inhaled substances. Our previous research demonstrated that DNA damage...