Simultaneous multi-residue analytical method for anesthetics and sedatives in seafood samples by LC-ESI/MSMS
•Simultaneous multi-residue analysis for 27 sedatives and anesthetics in seafood using LC-ESI/MSMS was developed.•The optimized pretreatment methods were extraction of flatfish and eel using ACN without any additives and extraction of shrimp using 0.1 % ammonium acetate in ACN.•The validation was sa...
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Published in | Food chemistry Vol. 404; no. Pt B; p. 134157 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
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Elsevier Ltd
15.03.2023
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Abstract | •Simultaneous multi-residue analysis for 27 sedatives and anesthetics in seafood using LC-ESI/MSMS was developed.•The optimized pretreatment methods were extraction of flatfish and eel using ACN without any additives and extraction of shrimp using 0.1 % ammonium acetate in ACN.•The validation was satisfied with CODEX Alimentarius (CAC/GL-71)
A simultaneous multi-residue analytical method for 27 regulated and unregulated anesthetics and sedatives in seafood using liquid chromatography/tandem mass spectrometry with electrospray ionization (LC-ESI/MSMS) was developed and tested on flatfish, eels, and shrimp. To optimize the efficiency of the method, extraction and clean-up procedures with various solvents and sorbents were tested. The most efficient pretreatment methods were extraction using acetonitrile (ACN) only (for flatfish and eel) and 0.1 % ammonium acetate in ACN (for shrimp). Validation was performed under the guidelines of CODEX Alimentarius (CAC/GL-71) and the Korean National Institute of Food and Drug Safety Evaluation (NIFDS). The limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision for all compounds ranged from 0.0002 to 0.002 mg/kg, 0.0005–0.005 mg/kg, 64.7–112.5 %, and 1.0–8.6 %, respectively. The coefficient of determination (linearity, R2) was over 0.98. Therefore, the method meets the requirements of both the domestic and international guidelines. |
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AbstractList | A simultaneous multi-residue analytical method for 27 regulated and unregulated anesthetics and sedatives in seafood using liquid chromatography/tandem mass spectrometry with electrospray ionization (LC-ESI/MSMS) was developed and tested on flatfish, eels, and shrimp. To optimize the efficiency of the method, extraction and clean-up procedures with various solvents and sorbents were tested. The most efficient pretreatment methods were extraction using acetonitrile (ACN) only (for flatfish and eel) and 0.1 % ammonium acetate in ACN (for shrimp). Validation was performed under the guidelines of CODEX Alimentarius (CAC/GL-71) and the Korean National Institute of Food and Drug Safety Evaluation (NIFDS). The limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision for all compounds ranged from 0.0002 to 0.002 mg/kg, 0.0005-0.005 mg/kg, 64.7-112.5 %, and 1.0-8.6 %, respectively. The coefficient of determination (linearity, R
) was over 0.98. Therefore, the method meets the requirements of both the domestic and international guidelines. •Simultaneous multi-residue analysis for 27 sedatives and anesthetics in seafood using LC-ESI/MSMS was developed.•The optimized pretreatment methods were extraction of flatfish and eel using ACN without any additives and extraction of shrimp using 0.1 % ammonium acetate in ACN.•The validation was satisfied with CODEX Alimentarius (CAC/GL-71) A simultaneous multi-residue analytical method for 27 regulated and unregulated anesthetics and sedatives in seafood using liquid chromatography/tandem mass spectrometry with electrospray ionization (LC-ESI/MSMS) was developed and tested on flatfish, eels, and shrimp. To optimize the efficiency of the method, extraction and clean-up procedures with various solvents and sorbents were tested. The most efficient pretreatment methods were extraction using acetonitrile (ACN) only (for flatfish and eel) and 0.1 % ammonium acetate in ACN (for shrimp). Validation was performed under the guidelines of CODEX Alimentarius (CAC/GL-71) and the Korean National Institute of Food and Drug Safety Evaluation (NIFDS). The limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision for all compounds ranged from 0.0002 to 0.002 mg/kg, 0.0005–0.005 mg/kg, 64.7–112.5 %, and 1.0–8.6 %, respectively. The coefficient of determination (linearity, R2) was over 0.98. Therefore, the method meets the requirements of both the domestic and international guidelines. |
ArticleNumber | 134157 |
Author | Muambo, Kimberly Etombi Nam, Ae-ji Moon, Haeran Oh, Jeong-Eun |
Author_xml | – sequence: 1 givenname: Haeran surname: Moon fullname: Moon, Haeran email: ran0678@pusan.ac.kr organization: Division of Civil Environmental Engineering, Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea – sequence: 2 givenname: Ae-ji surname: Nam fullname: Nam, Ae-ji email: tdn00117@pusan.ac.kr organization: Institute for Environmental and Energy, Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea – sequence: 3 givenname: Kimberly Etombi surname: Muambo fullname: Muambo, Kimberly Etombi email: kimmuambo2006@gmail.com organization: Division of Civil Environmental Engineering, Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea – sequence: 4 givenname: Jeong-Eun surname: Oh fullname: Oh, Jeong-Eun email: jeoh@pusan.ac.kr organization: Division of Civil Environmental Engineering, Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea |
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CitedBy_id | crossref_primary_10_1080_00032719_2024_2358160 crossref_primary_10_1016_j_jfca_2024_106000 crossref_primary_10_3390_foods12183422 |
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Keywords | LOD R2 Validation MRL Seafood d-SPE MRM ACN SLE LOQ Sedatives LC-ESI/MSMS Multi-residue analysis Liquid chromatography/tandem mass spectrometry with electrospray ionization mode S/N CV MeOH PLS NIFDS ID HPLC PSA HLB |
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Snippet | •Simultaneous multi-residue analysis for 27 sedatives and anesthetics in seafood using LC-ESI/MSMS was developed.•The optimized pretreatment methods were... A simultaneous multi-residue analytical method for 27 regulated and unregulated anesthetics and sedatives in seafood using liquid chromatography/tandem mass... |
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SubjectTerms | Anesthetics Animals Chromatography, Liquid Eels Hypnotics and Sedatives Liquid chromatography/tandem mass spectrometry with electrospray ionization mode Multi-residue analysis Seafood Sedatives Validation |
Title | Simultaneous multi-residue analytical method for anesthetics and sedatives in seafood samples by LC-ESI/MSMS |
URI | https://dx.doi.org/10.1016/j.foodchem.2022.134157 https://www.ncbi.nlm.nih.gov/pubmed/36444080 https://search.proquest.com/docview/2742657318 |
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