Vitrification of Mature Mouse Oocytes in a 6 M Me2SO Solution Supplemented with Antifreeze Glycoproteins: The Effect of Temperature

• Published in: Cryobiology Vol. 37; no. 1; pp. 59 - 66
• Main Authors: O'Neil, L., Paynter, S.J., Fuller, B.J., Shaw, R.W., DeVries, A.L.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.08.1998; Elsevier
• Subjects: Animals; antifreeze glycoproteins; Biological and medical sciences; Biological material (laboratory animals, preparation of biological tracers, etc.); Cryopreservation; Cryoprotective Agents; Dimethyl Sulfoxide; Embryology: invertebrates and vertebrates. Teratology; Female; Fundamental and applied biological sciences. Psychology; General aspects; General aspects. Development. Fetal membranes; Mice; mouse oocyte; Oocytes; Temperature; vitrification; dimethyl sulfoxide; antifreeze glycoproteins; mouse oocyte; vitrification; Treatment efficiency; Vitrification; Rodentia; Fecundation; In vitro; Cryobiology; Vertebrata; Mammalia; Mouse; Cryopreservation; Dimethyl sulfoxide; Cryoprotective agent; Antifreeze protein; Viability; Oocyte

Oocytes have been successfully cryopreserved using rapid and slow freezing procedures. However, variability in the success of replicates has limited its practical application. In the present study, mature mouse oocytes were vitrified in 6 M dimethyl sulfoxide supplemented with 1 mg/ml antifreeze gly...