Purification and partial characterization of three forms of alpha-glucosidase from the fruit fly Drosophila melanogaster

• Published in: Journal of biochemistry (Tokyo) Vol. 85; no. 1; pp. 123 - 130
• Main Authors: TANIMURA, Teiichi, KITAMLIRA, Keisuke, FUKUDA, Tomoko, KIKUCHI, Toshihide
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 1979
• Subjects: alpha-Glucosidases - isolation & purification; alpha-Glucosidases - metabolism; Animals; Drosophila melanogaster - enzymology; Glucosidases - isolation & purification; Isoenzymes - isolation & purification; Isoenzymes - metabolism; Molecular Weight

Three forms of β-glucosidase, I, II, and Ill, have been purified from the whole body extract of adult flies of Drosophila melanogaster in yields of 2·1, 5·3, and 6·7%, respectively. The purification procedures involved ammonium sulfate fractionation, Con A-Sepharose 4B affinity chromatography, DEAE-...