DETERMINATION OF CLONAZEPAM IN HAIR AND NAIL USING LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC-MS/MS)

Clonazepam, (5-(2-Chlorophenyl)-7-nitro-1,3-dihydro-1,4-benzodiazepin-2-one), a nitrobenzodiazepine and a scheduled drug, is one of the most highly abused drugs in recent times. A sensitive analytical methodology using Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) was developed for the d...

Full description

Saved in:
Bibliographic Details
Published inJournal of liquid chromatography & related technologies Vol. 37; no. 14; pp. 1917 - 1928
Main Authors John, Cijo, Ghosh, Priyankar, Varshney, K. M., Kaur, Sukhminder, Shukla, S. K., Satyanarayana, S.
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis Group 27.08.2014
Taylor & Francis Ltd
Subjects
Chloroform
Chromatography
clonazepam
Decontamination
Drug abuse
Drugs
Extraction
forensics
Hair
LC-MS/MS
Liquid chromatography
Mass spectrometry
Methyl alcohol
nail
Nails
Sodium dodecyl sulfate
Online AccessGet full text

Cover

Abstract Clonazepam, (5-(2-Chlorophenyl)-7-nitro-1,3-dihydro-1,4-benzodiazepin-2-one), a nitrobenzodiazepine and a scheduled drug, is one of the most highly abused drugs in recent times. A sensitive analytical methodology using Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) was developed for the determination of clonazepam in human hair and nail samples. The method involves the decontamination of hair and nail using 2 mL each of 0.1% SDS (sodium dodecyl sulfate), water, and methanol, dried followed by incubation in phosphate buffer (pH = 8), extraction with diethyl ether:chloroform (70:30, v/v), separation, and detection using liquid chromatography-tandem mass spectrometry. The method was totally validated with the limit of detection (LOD) of clonazepam estimated as 0.50pg/mg and the limit of quantification (LOQ), 2.0pg/mg of hair and nail. Linearity was observed in the range 2.0 pg/mg to 5000 pg/mg with r2 > 0.9980. The extraction recoveries, intra-day and inter-day precisions, accuracy, and matrix effect were also studied for the developed method. The amount of clonazepam detected in the hair and nail of different subjects was found to be in the range of 0.1156-1.040 ng/mg and 0.0713-0.880 ng/mg, respectively. The method is highly sensitive, precise, and reliable for the determination of clonazepam in hair and nail, which can be adopted by law enforcement laboratories for routine analysis.
AbstractList Clonazepam, (5-(2-Chlorophenyl)-7-nitro-1,3-dihydro-1,4-benzodiazepin-2-one), a nitrobenzodiazepine and a scheduled drug, is one of the most highly abused drugs in recent times. A sensitive analytical methodology using Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) was developed for the determination of clonazepam in human hair and nail samples. The method involves the decontamination of hair and nail using 2 mL each of 0.1% SDS (sodium dodecyl sulfate), water, and methanol, dried followed by incubation in phosphate buffer (pH = 8), extraction with diethyl ether:chloroform (70:30, v/v), separation, and detection using liquid chromatography-tandem mass spectrometry. The method was totally validated with the limit of detection (LOD) of clonazepam estimated as 0.50pg/mg and the limit of quantification (LOQ), 2.0pg/mg of hair and nail. Linearity was observed in the range 2.0 pg/mg to 5000 pg/mg with r2 > 0.9980. The extraction recoveries, intra-day and inter-day precisions, accuracy, and matrix effect were also studied for the developed method. The amount of clonazepam detected in the hair and nail of different subjects was found to be in the range of 0.1156-1.040 ng/mg and 0.0713-0.880 ng/mg, respectively. The method is highly sensitive, precise, and reliable for the determination of clonazepam in hair and nail, which can be adopted by law enforcement laboratories for routine analysis. [PUBLICATION ABSTRACT]
Clonazepam, (5-(2-Chlorophenyl)-7-nitro-1,3-dihydro-1,4-benzodiazepin-2-one), a nitrobenzodiazepine and a scheduled drug, is one of the most highly abused drugs in recent times. A sensitive analytical methodology using Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) was developed for the determination of clonazepam in human hair and nail samples. The method involves the decontamination of hair and nail using 2 mL each of 0.1% SDS (sodium dodecyl sulfate), water, and methanol, dried followed by incubation in phosphate buffer (pH = 8), extraction with diethyl ether:chloroform (70:30, v/v), separation, and detection using liquid chromatography-tandem mass spectrometry. The method was totally validated with the limit of detection (LOD) of clonazepam estimated as 0.50pg/mg and the limit of quantification (LOQ), 2.0pg/mg of hair and nail. Linearity was observed in the range 2.0 pg/mg to 5000 pg/mg with r2 > 0.9980. The extraction recoveries, intra-day and inter-day precisions, accuracy, and matrix effect were also studied for the developed method. The amount of clonazepam detected in the hair and nail of different subjects was found to be in the range of 0.1156-1.040 ng/mg and 0.0713-0.880 ng/mg, respectively. The method is highly sensitive, precise, and reliable for the determination of clonazepam in hair and nail, which can be adopted by law enforcement laboratories for routine analysis.
Clonazepam, (5-(2-Chlorophenyl)-7-nitro-1,3-dihydro-1,4-benzodiazepin-2-one), a nitrobenzodiazepine and a scheduled drug, is one of the most highly abused drugs in recent times. A sensitive analytical methodology using Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) was developed for the determination of clonazepam in human hair and nail samples. The method involves the decontamination of hair and nail using 2 mL each of 0.1% SDS (sodium dodecyl sulfate), water, and methanol, dried followed by incubation in phosphate buffer (pH = 8), extraction with diethyl ether:chloroform (70:30, v/v), separation, and detection using liquid chromatography-tandem mass spectrometry. The method was totally validated with the limit of detection (LOD) of clonazepam estimated as 0.50pg/mg and the limit of quantification (LOQ), 2.0pg/mg of hair and nail. Linearity was observed in the range 2.0 pg/mg to 5000 pg/mg with r2 > 0.9980. The extraction recoveries, intra-day and inter-day precisions, accuracy, and matrix effect were also studied for the developed method. The amount of clonazepam detected in the hair and nail of different subjects was found to be in the range of 0.1156-1.040 ng/mg and 0.0713-0.880 ng/mg, respectively. The method is highly sensitive, precise, and reliable for the determination of clonazepam in hair and nail, which can be adopted by law enforcement laboratories for routine analysis.
Author Satyanarayana, S.
Varshney, K. M.
John, Cijo
Kaur, Sukhminder
Ghosh, Priyankar
Shukla, S. K.
Author_xml – sequence: 1
  givenname: Cijo
  surname: John
  fullname: John, Cijo
  email: cijojohn01@gmail.com
  organization: Central Forensic Science Laboratory, Govt. of India
– sequence: 2
  givenname: Priyankar
  surname: Ghosh
  fullname: Ghosh, Priyankar
  organization: Central Forensic Science Laboratory, Govt. of India
– sequence: 3
  givenname: K. M.
  surname: Varshney
  fullname: Varshney, K. M.
  organization: Central Forensic Science Laboratory, Govt. of India
– sequence: 4
  givenname: Sukhminder
  surname: Kaur
  fullname: Kaur, Sukhminder
  organization: Central Forensic Science Laboratory, Govt. of India
– sequence: 5
  givenname: S. K.
  surname: Shukla
  fullname: Shukla, S. K.
  organization: Central Forensic Science Laboratory, Govt. of India
– sequence: 6
  givenname: S.
  surname: Satyanarayana
  fullname: Satyanarayana, S.
  organization: Department of Chemistry , Osmania University
BookMark eNqFkU1r3DAQhkVJoUnaf9CDoJf04I0-_KHtpQivsyvwx9b2QtOLUBQZHLxWKnkp-fe12faSQ3uZGZjnGQbeK3Ax2tEA8BGjFUYM3c6FxCiJVwRhumIkYhF-Ay5xRFAQJeT7xTzPSLAw78CV908I4SQi0SUYNlmb1YUoeSuqElZ3MM2rkv_I9ryAooQ7LmrIyw0sucjhoRHlFubi20FsYLqrq4K31bbm-909bGcqK2DBmwY2-yxt523W1vfwJk-Dorktms_vwdtODd58-NOvweEua9NdkFdbkfI80DRmU6AfY90pY4yKH0ITM2oQ1mHCiFIJUoZRHFJGugTFSq1RSJheo7XBpHsIiaJG0Wtwc7777OzPk_GTPPZem2FQo7EnLzFDi0boekY_vUKf7MmN83cSR5gSwjBZqPBMaWe9d6aTz64_KvciMZJLBPJvBHKJQJ4jmLUvrzTdT2rq7Tg51Q__k7-e5X7srDuqX9YNj3JSL4N1nVOj7r2k_7zwGzHplnw
CitedBy_id crossref_primary_10_52711_0974_360X_2023_00765
crossref_primary_10_1093_jat_bkv042
crossref_primary_10_52711_0974_360X_2022_00868
crossref_primary_10_1093_jat_bkac024
crossref_primary_10_52711_0974_360X_2022_00848
crossref_primary_10_1080_10826076_2015_1091008
crossref_primary_10_52711_0974_4150_2024_00051
crossref_primary_10_52711_0974_360X_2023_00108
Cites_doi 10.1016/j.forsciint.2011.10.024
10.1016/S1386-1425(03)00300-7
10.1002/jms.599
10.1016/j.jchromb.2004.12.036
10.1111/j.0013-9580.2004.01904.x
10.1176/ajp.145.12.1501
10.1016/j.forsciint.2010.04.046
10.1111/j.1528-1157.1983.tb04883.x
10.1016/j.forsciint.2004.04.022
10.1016/S1570-0232(04)00640-3
10.3928/0048-5713-19950301-08
10.1007/BF01981507
10.1016/0378-4347(96)00121-1
10.1093/jat/24.7.614
10.1016/j.forsciint.2004.04.066
10.1016/0378-4347(95)00017-D
10.1016/j.forsciint.2006.03.027
10.1111/j.1600-0404.2008.01004.x
10.1093/jat/15.5.260
10.1016/S0378-4347(96)00320-9
10.1016/S0378-4347(99)00059-6
10.1016/S0378-4347(98)00168-6
10.1016/S0378-4347(98)00391-0
10.1016/j.forsciint.2005.04.021
10.1016/0379-0738(84)90146-4
10.1365/s10337-008-0524-7
10.1016/S0379-0738(01)00511-4
ContentType Journal Article
Copyright Copyright Taylor & Francis Group, LLC 2014
Copyright Taylor & Francis Group, LLC
Copyright_xml – notice: Copyright Taylor & Francis Group, LLC 2014
– notice: Copyright Taylor & Francis Group, LLC
DBID AAYXX
CITATION
7QF
7QO
7QQ
7SE
7SR
7TB
7U5
7U7
8BQ
8FD
C1K
FR3
H8G
JG9
KR7
L7M
P64
DOI 10.1080/10826076.2013.825851
DatabaseName CrossRef
Aluminium Industry Abstracts
Biotechnology Research Abstracts
Ceramic Abstracts
Corrosion Abstracts
Engineered Materials Abstracts
Mechanical & Transportation Engineering Abstracts
Solid State and Superconductivity Abstracts
Toxicology Abstracts
METADEX
Technology Research Database
Environmental Sciences and Pollution Management
Engineering Research Database
Copper Technical Reference Library
Materials Research Database
Civil Engineering Abstracts
Advanced Technologies Database with Aerospace
Biotechnology and BioEngineering Abstracts
DatabaseTitle CrossRef
Materials Research Database
Civil Engineering Abstracts
Aluminium Industry Abstracts
Technology Research Database
Toxicology Abstracts
Mechanical & Transportation Engineering Abstracts
Ceramic Abstracts
METADEX
Biotechnology and BioEngineering Abstracts
Environmental Sciences and Pollution Management
Copper Technical Reference Library
Engineered Materials Abstracts
Biotechnology Research Abstracts
Solid State and Superconductivity Abstracts
Engineering Research Database
Corrosion Abstracts
Advanced Technologies Database with Aerospace
DatabaseTitleList Materials Research Database

Technology Research Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
EISSN 1520-572X
EndPage 1928
ExternalDocumentID 3267869551
10_1080_10826076_2013_825851
825851
Genre Feature
GroupedDBID ---
-~X
.7F
.QJ
0BK
0R~
29K
30N
4.4
5GY
5VS
A8Z
AAENE
AAJMT
AALDU
AAMIU
AAPUL
AAQRR
ABCCY
ABCQX
ABDBF
ABFIM
ABHAV
ABJNI
ABLIJ
ABPAQ
ABPEM
ABTAI
ABXUL
ABXYU
ACGEJ
ACGFS
ACIWK
ACPRK
ACTIO
ACUHS
ADCVX
ADGTB
ADXPE
AEISY
AENEX
AEOZL
AEPSL
AEYOC
AFKVX
AFRAH
AGDLA
AGMYJ
AHDZW
AIJEM
AJWEG
AKBVH
AKOOK
ALMA_UNASSIGNED_HOLDINGS
ALQZU
AQRUH
AVBZW
AWYRJ
BLEHA
CCCUG
CE4
CS3
D-I
DGEBU
DKSSO
DU5
EAP
EAS
EBD
EBS
EJD
EMK
EPL
EST
ESTFP
ESX
E~A
E~B
F5P
GTTXZ
H13
HF~
HZ~
H~P
I-F
IPNFZ
J.P
KYCEM
L7B
M4Z
NA5
NW0
O9-
P2P
RIG
RNANH
ROSJB
RTWRZ
S-T
SNACF
TBQAZ
TCY
TDBHL
TFL
TFT
TFW
TTHFI
TUROJ
TUS
TWF
UT5
UU3
ZGOLN
~S~
AAGDL
AAHIA
AAYXX
ADYSH
AFRVT
AIYEW
AMPGV
CITATION
7QF
7QO
7QQ
7SE
7SR
7TB
7U5
7U7
8BQ
8FD
C1K
FR3
H8G
JG9
KR7
L7M
P64
TASJS
ID FETCH-LOGICAL-c368t-cd6cfaeeea6b4e683e01c4782aa70ae8314382f706aa90428c909e12fb42a3ea3
ISSN 1082-6076
IngestDate Fri Jul 11 13:06:26 EDT 2025
Wed Aug 13 11:24:10 EDT 2025
Thu Apr 24 22:56:44 EDT 2025
Tue Jul 01 00:46:12 EDT 2025
Wed Dec 25 09:06:38 EST 2024
IsPeerReviewed true
IsScholarly true
Issue 14
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c368t-cd6cfaeeea6b4e683e01c4782aa70ae8314382f706aa90428c909e12fb42a3ea3
Notes SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 14
ObjectType-Article-1
ObjectType-Feature-2
content type line 23
PQID 1513228129
PQPubID 186332
PageCount 12
ParticipantIDs crossref_primary_10_1080_10826076_2013_825851
crossref_citationtrail_10_1080_10826076_2013_825851
informaworld_taylorfrancis_310_1080_10826076_2013_825851
proquest_miscellaneous_1800428239
proquest_journals_1513228129
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2014-08-27
PublicationDateYYYYMMDD 2014-08-27
PublicationDate_xml – month: 08
  year: 2014
  text: 2014-08-27
  day: 27
PublicationDecade 2010
PublicationPlace Abingdon
PublicationPlace_xml – name: Abingdon
PublicationTitle Journal of liquid chromatography & related technologies
PublicationYear 2014
Publisher Taylor & Francis Group
Taylor & Francis Ltd
Publisher_xml – name: Taylor & Francis Group
– name: Taylor & Francis Ltd
References CIT0030
CIT0010
CIT0031
CIT0012
CIT0034
CIT0011
CIT0033
Ciraulo D. A. (CIT0007) 1988; 145
Dumont R. L. (CIT0009) 1988; 14
CIT0014
CIT0013
CIT0016
Longo L. P. (CIT0006) 2000; 61
CIT0015
Cloos J.-M. (CIT0004) 2005; 18
CIT0018
CIT0017
CIT0019
Kintz P. (CIT0032) 1996
CIT0021
CIT0020
CIT0001
CIT0023
CIT0022
Roache J. D. (CIT0008) 1995; 25
CIT0003
CIT0025
CIT0002
CIT0024
CIT0005
CIT0027
CIT0026
CIT0029
CIT0028
References_xml – ident: CIT0029
  doi: 10.1016/j.forsciint.2011.10.024
– volume: 14
  start-page: 1
  year: 1988
  ident: CIT0009
  publication-title: Am. J. Drug Alcohol Abuse
– ident: CIT0016
  doi: 10.1016/S1386-1425(03)00300-7
– ident: CIT0025
  doi: 10.1002/jms.599
– ident: CIT0024
  doi: 10.1016/j.jchromb.2004.12.036
– volume: 18
  start-page: 45
  issue: 1
  year: 2005
  ident: CIT0004
  publication-title: Curr. Opin. Psych.
– ident: CIT0002
  doi: 10.1111/j.0013-9580.2004.01904.x
– volume: 145
  start-page: 1501
  year: 1988
  ident: CIT0007
  publication-title: Am. J. Psych.
  doi: 10.1176/ajp.145.12.1501
– ident: CIT0026
  doi: 10.1016/j.forsciint.2010.04.046
– ident: CIT0003
  doi: 10.1111/j.1528-1157.1983.tb04883.x
– ident: CIT0031
– ident: CIT0028
  doi: 10.1016/j.forsciint.2004.04.022
– ident: CIT0023
  doi: 10.1016/S1570-0232(04)00640-3
– volume: 25
  start-page: 153
  issue: 3
  year: 1995
  ident: CIT0008
  publication-title: Psych. Annal
  doi: 10.3928/0048-5713-19950301-08
– ident: CIT0033
  doi: 10.1007/BF01981507
– ident: CIT0018
  doi: 10.1016/0378-4347(96)00121-1
– ident: CIT0027
  doi: 10.1093/jat/24.7.614
– ident: CIT0021
  doi: 10.1016/j.forsciint.2004.04.066
– ident: CIT0017
  doi: 10.1016/0378-4347(95)00017-D
– ident: CIT0014
  doi: 10.1016/j.forsciint.2006.03.027
– ident: CIT0005
  doi: 10.1111/j.1600-0404.2008.01004.x
– ident: CIT0034
  doi: 10.1093/jat/15.5.260
– ident: CIT0012
  doi: 10.1016/S0378-4347(96)00320-9
– ident: CIT0010
  doi: 10.1016/S0378-4347(99)00059-6
– ident: CIT0011
  doi: 10.1016/S0378-4347(98)00168-6
– ident: CIT0001
– ident: CIT0019
  doi: 10.1016/S0378-4347(98)00391-0
– ident: CIT0022
  doi: 10.1016/j.forsciint.2005.04.021
– ident: CIT0030
– ident: CIT0013
  doi: 10.1016/0379-0738(84)90146-4
– volume: 61
  start-page: 2121
  year: 2000
  ident: CIT0006
  publication-title: Am. Family Phys.
– ident: CIT0015
  doi: 10.1365/s10337-008-0524-7
– start-page: 267
  volume-title: Drug Testing in Hair
  year: 1996
  ident: CIT0032
– ident: CIT0020
  doi: 10.1016/S0379-0738(01)00511-4
SSID ssj0017525
Score 2.0325084
Snippet Clonazepam, (5-(2-Chlorophenyl)-7-nitro-1,3-dihydro-1,4-benzodiazepin-2-one), a nitrobenzodiazepine and a scheduled drug, is one of the most highly abused...
SourceID proquest
crossref
informaworld
SourceType Aggregation Database
Enrichment Source
Index Database
Publisher
StartPage 1917
SubjectTerms Chloroform
Chromatography
clonazepam
Decontamination
Drug abuse
Drugs
Extraction
forensics
Hair
LC-MS/MS
Liquid chromatography
Mass spectrometry
Methyl alcohol
nail
Nails
Sodium dodecyl sulfate
Title DETERMINATION OF CLONAZEPAM IN HAIR AND NAIL USING LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC-MS/MS)
URI https://www.tandfonline.com/doi/abs/10.1080/10826076.2013.825851
https://www.proquest.com/docview/1513228129
https://www.proquest.com/docview/1800428239
Volume 37
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3dc9JAEL9B-qAvjp9ja3XOGR90Mmkhl8_HTAhNLAGkQWlfMpfkGLAISsmD_kH-ne7lkhBG1OpLBpILF25_2fvt3t4uQq8VBd4wTdPkRKOGrCrTWKbEimXD0hKiWkasJXzvcNDXvbH6bqJNGo0ftailbBOfJN_37iv5H6nCOZAr3yX7D5KtfhROwGeQLxxBwnC8lYw7LpDRwBcZbXn8jtMb9O0rd2gHkt-XPNsf5emj-rbfk3iBjTNQVu_HfkdyvNEgsMPB2cgeepdSCK3cQApsXoBj6DohXHXD0WVe8MORgwt4Rn6wfsNlF_Ov2TyVktl6BQy4yIKdoyrfKwOkdlO68OtBi6uZWPKff1pVcUCz1Y3w9Kzn3-jymlbBwx_ABJ8VsWnnWyfuOc1E6bDsevaZ535c1x0ZbZV7ZkVegEL3AhuR9ZZRZMYu9DFYt5qRV1yvFDYx6sBUa-qXG5-1qRzYq7l3mhBxlbxD3h8P8CMnYCqbRe7bnazc_UHUHfd6UehOwjvoQAFzRGmiA9vrXH2s1qsMLS_vW_2FcpOm2Trd18sOCdpJkfsLJch5TvgA3S-Eim2BtoeowZaP0F2nrAv4GC12UIcHXbxFHfb7mKMOA54wRx3OUYcF6vAu6rBAHeaow3XU4Tc55k6Di7dP0Ljrho4nFzU75ITo5kZOUj2ZUsYY1WOV6SZhrXaiAg2l1GhRZpI2X3meGi2dUovb64nVslgbdISqUMIoeYqay9WSPUM4VWkSG6lmUJOoU1WjSprCN8VKLZ0ZsXWISDmIUVIktOd1VRZRu8h7Ww59xIc-EkN_iOTqri8ioctf2pt1-USb3JE2FVVvIvLnW49LWUaF1riJgGHDHAq0Gp7_VXUZRMgX6uiSrTJoY-auDIVYR7do8xzd275Ox6i5WWfsBTDlTfyygOlPKoKmvw
linkProvider Library Specific Holdings
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3Nj5QwFG_MelgvfhtXV62JBz10ZGhL6ZEwzIICMw5M4nohpZSLm1njMhf_el_5mLgaNdETIe2D0r4-fq99fT-EXrkuzDDOOdFcCcLctiaKypoIyTVlUtRc27PDWe7FW_buI5-iCa_GsErrQ7dDoojeVtvJbRejp5A4uAIoBgfcRmbRGfg4vj1EfZNLT1gSA-rkh40EwXveVStBrMh0eu43T7n2d7qWu_QXW93_gJZ3UD01fYg7-Tzbd_VMf_spq-N_fdtddHuEpzgY9OkeumF299FxOLHCPUAXiwgAcJYMWXTxaonDdJUHn6J1kOEkx3GQbHCQL3AeJCm2pB5nOE0-bJMFDuPNKgvK1dkmWMfnuIRaUYazoChwsY7CEkqjcnOOX6chyYq3WfHmIdouozKMycjYQDT1_I7oxtOtMsYor2bG86lx5poBCFFKOMr41JKtu61wPKWk9da0dKSZg4YwV1Gj6CN0tLvcmccIN0zpWjRcKJ-ylnHlNg3cubKRnhG1PEF0GqlKj-nMLavGRTUfs55OPVnZnqyGnjxB5CD1ZUjn8Zf6_o9KUHX9Mko7cJ5U9M-ip5PCVKNduKoAX4EFBVAF7X95KIYhtNs0amcu91DH7x1Zl8on__72F-g4LrO0SpP8_VN0C0qYXQl3xSk66r7uzTOAUl39vJ8s3wEvaAWg
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELZQkYALb0RLASNxgIOXbGzH8THKZptAkl02u1LLJXIc50K1rUr2wq9nnMeqBQESnKLInsSP8XjGnvkGobeuCyuMc040V4Iwt6mIorIiQnJNmRQV1zZ2OMu9eMM-nvLTa1H81q3S2tBNDxTRyWq7uC_rZvSIgyfoxGB_W8csOgETx7cx1Lc9ix1ugzicfH-PIHiXdtVSEEsyBs_95is3Nqcb0KW_iOpu_5k_QGpsee928nWya6uJ_v4TqOP_dO0huj8opzjouekRumW2j9HdcMwJ9wSdzyJQf7Okx9DFizkO00UefImWQYaTHMdBssJBPsN5kKTYpvQ4wWnyeZPMcBivFlmwXpysgmV8htdQK8pwFhQFLpZRuIbSaL06w-_SkGTFh6x4_xRt5tE6jMmQr4Fo6vkt0bWnG2WMUV7FjOdT40w1AxVEKeEo41Obat1thOMpJa2tpqUjzRT4g7mKGkWfoYPtxdY8R7hmSlei5kL5lDWMK7eu4c2VtfSMqOQhouNElXoAM7c5Nc7L6YB5Oo5kaUey7EfyEJE91WUP5vGX-v51Hijb7hCl6TOelPTPpMcjv5SDVPhWgnYF8hNUKmj_m30xTKG9pFFbc7GDOn5nxrpUHv3731-jO8vZvEyT_NMLdA8KmD0Gd8UxOmivduYl6FFt9apbKj8Ayh0ERA
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=DETERMINATION+OF+CLONAZEPAM+IN+HAIR+AND+NAIL+USING+LIQUID+CHROMATOGRAPHY+TANDEM+MASS+SPECTROMETRY+%28LC-MS%2FMS%29&rft.jtitle=Journal+of+liquid+chromatography+%26+related+technologies&rft.au=John%2C+Cijo&rft.au=Ghosh%2C+Priyankar&rft.au=Varshney%2C+K+M&rft.au=Kaur%2C+Sukhminder&rft.date=2014-08-27&rft.issn=1082-6076&rft.eissn=1520-572X&rft.volume=37&rft.issue=14&rft.spage=1917&rft.epage=1928&rft_id=info:doi/10.1080%2F10826076.2013.825851&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1082-6076&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1082-6076&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1082-6076&client=summon