Distance determination between low-spin ferric haem and nitroxide spin label using DEER: the neuroglobin case

• Published in: Molecular physics Vol. 111; no. 18-19; pp. 2855 - 2864
• Main Authors: Ezhevskaya, M., Bordignon, E., Polyhach, Y., Moens, L., Dewilde, S., Jeschke, G., Van Doorslaer, S.
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01.10.2013; Taylor & Francis Ltd
• Subjects: Deer; distance measurements; EPR; neuroglobin

This work demonstrates for the first time the feasibility of using double electron-electron resonance (DEER) to determine the inter-spin distance between nitroxide spin labels and low-spin (S = 1/2) ferric haem centres. For these means, two human neuroglobin variants were spin labelled leading to si...