Tumor necrosis factor mediation of NSAID-induced gastric damage: role of leukocyte adherence

• Published in: The American journal of physiology Vol. 270; no. 1 Pt 1; p. G42
• Main Authors: Appleyard, C B, McCafferty, D M, Tigley, A W, Swain, M G, Wallace, J L
• Format: Journal Article
• Language: English
• Published: United States 01.01.1996
• Subjects: Animal Feed; Animals; Anti-Inflammatory Agents, Non-Steroidal; Cell Adhesion; Cell Movement; Gastrointestinal Hemorrhage - chemically induced; Gastrointestinal Hemorrhage - pathology; Indomethacin; Leukocytes - physiology; Male; Naproxen; Rats; Rats, Wistar; Stomach - pathology; Stomach Diseases - chemically induced; Stomach Diseases - pathology; Stomach Diseases - physiopathology; Stomach Ulcer - chemically induced; Stomach Ulcer - pathology; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - physiology
• DOI: 10.1152/ajpgi.1996.270.1.g42
• ISSN: 0002-9513

Neutrophil adherence to the vascular endothelium has been suggested to be a critical event in the pathogenesis of nonsteroidal anti-inflammatory drug (NSAID)-induced gastric damage. Recently, increased plasma levels of tumor necrosis factor-alpha (TNF-alpha), which can increase leukocyte adherence, have been reported after administration of indomethacin. This study was performed to determine the relationship between plasma TNF-alpha levels, leukocyte adherence, and NSAID-induced gastric injury. Administration of indomethacin to rats resulted in a significant elevation of plasma TNF-alpha levels within 30 min and the development of gastric erosions. Pretreatment with dexamethasone and prostaglandin E2 almost completely prevented gastric injury and abolished the rise in plasma TNF-alpha. Pentoxifylline dose dependently reduced both gastric damage and plasma TNF-alpha. Similar effects were observed with three other TNF-alpha synthesis inhibitors and with an anti-TNF-alpha antisera. Pentoxifylline also significantly reduced the extent of antral ulceration induced by naproxen. However, pentoxifylline did not significantly affect indomethacin-induced leukocyte adherence. These results suggest that TNF-alpha plays a critical role in the pathogenesis of NSAID-induced gastric injury, but this cytokine may not be responsible for NSAID-induced leukocyte adherence.