Purification of rabbit skeletal muscle proteoglycogen: studies on the glucosyltransferase activity of polysaccharide-free and -bound glycogenin

• Published in: Glycobiology (Oxford) Vol. 7; no. 4; pp. 571 - 578
• Main Authors: Carrizo, Maria E., Miozzo, Maria C., Goldraij, Ariel, Curtino, Juan A.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.06.1997
• Subjects: Animals; Chemical Precipitation; Chromatography, Ion Exchange; Detergents; Glucosyltransferases - metabolism; Glycogen - metabolism; glycogenin; glycogenin autogalactosylation; Glycoproteins - chemistry; Glycoproteins - isolation & purification; Glycoproteins - metabolism; Glycosylation; Molecular Weight; Muscle Proteins - isolation & purification; Muscle Proteins - metabolism; Muscle, Skeletal - chemistry; Polysaccharides - metabolism; Potassium Iodide; proteoglycogen; proteoglycogen activity; proteoglycogen isolation; Rabbits; Solubility; Uridine Diphosphate Glucose - metabolism

Proteoglycogen is the end product in the process of glycogen biogenesis. We have purified rabbit muscle proteoglycogen and studied the glucosyltransferase reactions catalyzed by its protein moiety, glycogenin, free or bound to the polysaccharide. The purification strategy involved dissolution of pro...