High production of poly(3-hydroxybutyrate) in Escherichia coli using crude glycerol

• Published in: Bioresource technology Vol. 384; p. 129315
• Main Authors: Ta, Doan-Thanh, Chiang, Chung-Jen, Huang, Zhu-Xuan, Luu, Nguyen-Luan, Chao, Yun-Peng
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2023
• Subjects: 3-Hydroxybutyric Acid - metabolism; Crude glycerol; Escherichia coli - genetics; Escherichia coli - metabolism; Glycerol - metabolism; Hydroxybutyrates - metabolism; Metabolic engineering; Plastics - metabolism; Poly(3-hydroxybutyrate); Polyesters - metabolism; PP; TCA; CDW; Poly(3-hydroxybutyrate); PHA; Metabolic engineering; Crude glycerol; PHB; DO

[Display omitted] •Development of the PHB-producing Escherichia coli strain based on crude glycerol.•Improvement of the producer strain by rewiring central metabolism.•Implementation of fed-batch fermentation for high PHB titer, content, and productivity. Poly(3-hydroxybutyrate) (PHB) is a prominent...