Beneficial effects of rolipram, a phosphodiesterase 4 specific inhibitor, on testicular torsion-detorsion injury in rats

• Published in: Journal of pediatric surgery Vol. 53; no. 11; pp. 2261 - 2265
• Main Authors: Abat, Deniz, Bayazıt, Yıldırım, Açıkalın, Arbil, Dağlıoğlu, Kenan, Yenilmez, Ebru Dündar, Altunkol, Adem, Erdoğan, Şeyda, Tuli, Abdullah
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2018
• Subjects: Animals; Apoptosis - drug effects; Ischaemia/reperfusion injury; Male; Malondialdehyde - analysis; Necrosis - pathology; Nitric Oxide - analysis; Phosphodiesterase 4 Inhibitors - administration & dosage; Phosphodiesterase 4 Inhibitors - pharmacology; Rats; Reactive oxygen species; Rolipram - administration & dosage; Rolipram - pharmacology; Spermatic Cord Torsion; Testis; Testis - chemistry; Testis - drug effects; Testis - pathology; Testis; Ischaemia/reperfusion injury; Reactive oxygen species
• DOI: 10.1016/j.jpedsurg.2018.04.013
• ISSN: 0022-3468

The aim of the study is to investigate the effect of Rolipram, a selective phosphodiesterase 4 inhibitor, on testicular torsion – detorsion injury. Sixty young male rats were divided into five groups. In each group, the right testes of six rats were removed four hours after detorsion for biochemical analysis, and the right testes of the remaining six rats were removed 24 h after detorsion for pathological analysis. In group 1 (sham-operated) right orchiectomy was performed without torsion, and right testes were sent to the laboratory for biochemical and pathologic analyses. In group 2 (control) torsion was applied to the right testes for 60 min, and detorsion was performed without the administration of Rolipram. In group 3 torsion was applied to the right testes for 60 min. 1 mg/kg Rolipram was administered 30 min before detorsion. In group 4 torsion was applied to the right testes for 60 min, and 1 mg/kg Rolipram was administered during detorsion. In group 5 torsion was applied to the right testes for 60 min. 1 mg/kg Rolipram was administered 30 min after detorsion. The malondialdehyde and nitric oxide levels were determined. The rates of necrosis and apoptosis were evaluated by histopathological examination. The level of malondialdehyde was higher in the torsioned groups (Group 2, 3, 4, 5) than that in group 1 (p = 0.004). There was no statistically significant difference between the groups regarding the level of nitric oxide (p = 0.182). Apoptosis was higher in groups 2, 3 and 4 than in group 1; however, apoptosis was similar in group 1 and group 5 (p = 0.122). The level of necrosis in group 1 was similar to that in groups 4 and 5 (p = 0.194 and p = 0.847, respectively). We suggest that the administration of Rolipram can decrease the rate of necrosis and apoptosis in testicular ischaemia-reperfusion injury.