Pooling of Immunomagnetic Separation Beads Does Not Affect Detection Sensitivity of Six Major Serogroups of Shiga Toxin–Producing Escherichia coli in Cattle Feces

• Published in: Journal of food protection Vol. 79; no. 1; pp. 59 - 65
• Main Authors: Noll, Lance W., Baumgartner, William C., Shridhar, Pragathi B., Cull, Charley A., Dewsbury, Diana M., Shi, Xiaorong, Cernicchiaro, Natalia, Renter, David G., Nagaraja, T.G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Limited 01.01.2016
• Subjects: Animals; Asymptomatic; Beef cattle; Cattle; detection limit; E coli; Experiments; Feces; Feces - microbiology; Food contamination & poisoning; food pathogens; Food safety; foods; hindgut; immunomagnetic separation; Immunomagnetic Separation - methods; labor; Pathogens; Potassium; serotypes; Serotyping; Shiga Toxin - metabolism; Shiga toxin-producing Escherichia coli; Shiga-Toxigenic Escherichia coli - classification; Shiga-Toxigenic Escherichia coli - genetics; Shiga-Toxigenic Escherichia coli - isolation & purification; Shiga-Toxigenic Escherichia coli - metabolism; Toxins

Shiga toxin-producing Escherichia coli (STEC) of the serogroups O26, O45, O103, O111, O121, and O145, often called non-O157 STEC, are foodborne pathogens. Cattle are asymptomatic reservoirs for STEC; the organisms reside in the hindgut and are shed in the feces, which serve as the source of food pro...