The comet assay for the evaluation of gut content genotoxicity: Use in human studies as an early biomarker of colon cancer risk

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis Vol. 878; p. 503477
• Main Authors: Ristori, Sara, Scavone, Francesca, Bartolozzi, Chiara, Giovannelli, Lisa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2022
• Subjects: Animals; Biomarkers; Colonic Neoplasms - genetics; Comet Assay; CRC biomarker; ENDO III; Fecal water genotoxicity; Fpg; Humans; Nutrition; Water; ENDO III; Nutrition; Fpg; CRC biomarker; Fecal water genotoxicity

This short narrative review describes the use of the comet assay to evaluate the formation of genotoxic compounds in the gut lumen in human studies. The fecal water genotoxicity assay is based on ability of the gut content to induce genotoxicity in a cellular model, employing the aqueous component o...