Hydrophilic poly (ethylene glycol) capped poly (lactic-co-glycolic) acid nanoparticles for subcutaneous delivery of insulin in diabetic rats

• Published in: International journal of biological macromolecules Vol. 95; pp. 1190 - 1198
• Main Authors: S, Saravanan, S, Malathi, P S L, Sesh, S, Selvasubramanian, S, Balasubramanian, V, Pandiyan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2017
• Subjects: Animals; Blood Glucose - metabolism; Catalase - metabolism; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Drug Carriers; Drug Compounding; Drug Liberation; Emulsions; Glutathione Peroxidase - metabolism; Glycogen - metabolism; Hydrophobic and Hydrophilic Interactions; Hypoglycemic Agents - metabolism; Hypoglycemic Agents - pharmacokinetics; Hypoglycemic Agents - pharmacology; Injections, Subcutaneous; Insulin; Insulin - metabolism; Insulin - pharmacokinetics; Insulin - pharmacology; Lactic Acid - chemistry; Liver - drug effects; Liver - metabolism; Male; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Pancreas - drug effects; Pancreas - metabolism; Particle Size; Poly(ethylene glycol); Poly(lactic-co-glycolic)acid; Polyethylene Glycols - chemistry; Polyglycolic Acid - chemistry; Rats; Rats, Wistar; Streptozocin; Subcutaneous administration; Superoxide Dismutase - metabolism; Poly(lactic-co-glycolic)acid; Nanoparticles; Subcutaneous administration; Insulin; Poly(ethylene glycol)
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2016.11.009

[Display omitted] •Low molecular weight PLGA copolymers were synthesized by melt polycondensation.•The insulin loaded PEG capped PLGA NPs (ISPPLG NPs) were synthesized by water-oil-water emulsion solvent evaporation method.•The in-vivo studies of ISPPLG NPs carried out in diabetic rats by subcutaneous administration show considerable reduction in serum glucose level.•Histopathological studies reveal that ISPPLG NPs could restore the damages caused by oxidants during hyperglycaemia.•Synthesis of PEG capped PLGA Nps by water-oil-water emulsion solvent evaporation method. The aim of the present study is to evaluate the effect of insulin loaded poly(ethylene glycol) capped poly(lactic-co-glycolic)acid nanoparticles (ISPPLG NPs) by subcutaneous administration in diabetic rats. A series of biodegradable low molecular weight PLGA [90/10 (PLG2) and 80/20 (PLG4)] copolymers were synthesized by melt polycondensation and their ISPPLG NPs were synthesized by water-oil-water (W/O/W) emulsion solvent evaporation method. The PLGA copolymers and their nanoparticles were characterized. The maximum encapsulation efficiency of ISPPLG4 NPs is 66% and the diameter of the nanoparticles is about 140nm. The in-vivo studies of ISPPLG NPs carried out in diabetic rats by subcutaneous administration show considerable reduction in serum glucose level along with partial restoration of tissue defense systems. Histopathological studies reveal that ISPPLG NPs could restore the damages caused by oxidants during hyperglycaemia. The subcutaneous administration of ISPPLG4 NPs is thus an effective method of reducing hyperglycaemia associated complications.