Hemoglobin biosynthesis in individual erythropoietic bursts in culture. Studies of adult peripheral blood

By using a methylcellulose clonal assay, we cultured peripheral blood erythropoietic precursors from a patient with sickle cell anemia, a patient with sickle cell hemoglobin C disease, and a normal volunteer. We then analyzed the synthetic rates of adult and fetal hemoglobins (Hb) in individual eryt...

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Published inThe Journal of clinical investigation Vol. 63; no. 4; pp. 804 - 806
Main Authors Kidoguchi, K, Ogawa, M, Karam, J D
Format Journal Article
LanguageEnglish
Published United States 01.04.1979
Subjects
Adult
Anemia, Sickle Cell - blood
Cells, Cultured
Erythropoiesis
Fetal Hemoglobin - biosynthesis
Hemoglobin, Sickle - biosynthesis
Hemoglobins - biosynthesis
Humans
Kinetics
Monocytes - metabolism
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Abstract By using a methylcellulose clonal assay, we cultured peripheral blood erythropoietic precursors from a patient with sickle cell anemia, a patient with sickle cell hemoglobin C disease, and a normal volunteer. We then analyzed the synthetic rates of adult and fetal hemoglobins (Hb) in individual erythropoietic bursts. Bb were labeled with 14C-amino acids in culture, separated by slab gel isoelectric focusing techniques, and quantitated by fluorographic methods. All bursts exhibited both fetal and adult Hb in varying ratios. Frequency distributions of the individual burst differing in percentage of BbF biosynthesis approached normal distributions. Further stimulation of HbF synthesis by higher erythropoietin in culture was associated with increased HbF biosynthesis in individual bursts. Augmentation of human HbF synthesis in culture appears to be controlled by qualitative intracellular changes rather than by changes in cellular population.
AbstractList By using a methylcellulose clonal assay, we cultured peripheral blood erythropoietic precursors from a patient with sickle cell anemia, a patient with sickle cell hemoglobin C disease, and a normal volunteer. We then analyzed the synthetic rates of adult and fetal hemoglobins (Hb) in individual erythropoietic bursts. Bb were labeled with 14C-amino acids in culture, separated by slab gel isoelectric focusing techniques, and quantitated by fluorographic methods. All bursts exhibited both fetal and adult Hb in varying ratios. Frequency distributions of the individual burst differing in percentage of BbF biosynthesis approached normal distributions. Further stimulation of HbF synthesis by higher erythropoietin in culture was associated with increased HbF biosynthesis in individual bursts. Augmentation of human HbF synthesis in culture appears to be controlled by qualitative intracellular changes rather than by changes in cellular population.
Author Ogawa, M
Karam, J D
Kidoguchi, K
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Snippet By using a methylcellulose clonal assay, we cultured peripheral blood erythropoietic precursors from a patient with sickle cell anemia, a patient with sickle...
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SubjectTerms Adult
Anemia, Sickle Cell - blood
Cells, Cultured
Erythropoiesis
Fetal Hemoglobin - biosynthesis
Hemoglobin, Sickle - biosynthesis
Hemoglobins - biosynthesis
Humans
Kinetics
Monocytes - metabolism
Title Hemoglobin biosynthesis in individual erythropoietic bursts in culture. Studies of adult peripheral blood
URI https://www.ncbi.nlm.nih.gov/pubmed/438339
https://search.proquest.com/docview/74506630
https://pubmed.ncbi.nlm.nih.gov/PMC372018
Volume 63
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