High resolution characterization of soil biological communities by nucleic acid and fatty acid analyses

• Published in: Soil biology & biochemistry Vol. 34; no. 12; pp. 1853 - 1860
• Main Authors: Dierksen, Karen P, Whittaker, Gerald W, Banowetz, Gary M, Azevedo, Mark D, Kennedy, Ann C, Steiner, Jeffrey J, Griffith, Stephen M
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.12.2002; New York, NY Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Diversity; Fatty acid methyl ester; Fundamental and applied biological sciences. Psychology; Length heterogeneity PCR; Microbial ecology; MIDI-FAME; Nucleic acid analysis; Soil science; Soil surveys, classification and mapping; Soil surveys, classification and mapping, soil genesis; Statistics; Tillage; Oregon; United States; North America; America; MIDI-FAME; Length heterogeneity PCR; Tillage; Diversity; Microbial ecology; Fatty acid methyl ester; Statistics; Nucleic acid analysis; Monocotyledones; High resolution; Identification; Characterization; Gramineae; Angiospermae; Classification; Plant cover; Nucleic acid; Microbial community; Festuca arundinacea; Silty soil; Statistical analysis; Soil tillage; Mollisols; Fatty acids; Silt loam soil; Fingerprint method; Spermatophyta; Fodder crop; Measurement method

Fatty acid methyl ester (FAME) and length heterogeneity-polymerase chain reaction (LH-PCR) analyses were used to generate ‘fingerprints’ of FAMEs and eubacterial 16S rDNA sequences characteristic of agricultural soil communities. We hypothesized that pooling data from two methods that characterized...