Photoaffinity Labeling and Quantitative Chemical Proteomics Identify LXRβ as the Functional Target of Enhancers of Astrocytic apoE

• Published in: Cell chemical biology Vol. 28; no. 2; pp. 148 - 157.e7
• Main Authors: Seneviratne, Uthpala, Huang, Zhen, am Ende, Christopher W., Butler, Todd W., Cleary, Leah, Dresselhaus, Erica, Evrard, Edelweiss, Fisher, Ethan L., Green, Michael E., Helal, Christopher J., Humphrey, John M., Lanyon, Lorraine F., Marconi, Michael, Mukherjee, Paramita, Sciabola, Simone, Steppan, Claire M., Sylvain, Emily K., Tuttle, Jamison B., Verhoest, Patrick R., Wager, Travis T., Xie, Longfei, Ramaswamy, Gayathri, Johnson, Douglas S., Pettersson, Martin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 18.02.2021
• Subjects: apoE; CETSA; chemical proteomics; LXRβ; MOA; phenotypic screening; target identification; CETSA; target identification; apoE; phenotypic screening; chemical proteomics; MOA; LXRβ

Utilizing a phenotypic screen, we identified chemical matter that increased astrocytic apoE secretion in vitro. We designed a clickable photoaffinity probe based on a pyrrolidine lead compound and carried out probe-based quantitative chemical proteomics in human astrocytoma CCF-STTG1 cells to identi...