Chemometric Approach for RP-HPLC Determination of Azithromycin, Secnidazole, and Fluconazole Using Response Surface Methodology

• Published in: Journal of liquid chromatography & related technologies Vol. 38; no. 6; pp. 750 - 758
• Main Authors: Sahoo, Dillip Kumar, Sahu, Prafulla Kumar
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 03.04.2015; Taylor & Francis Ltd
• Subjects: Arrays; azithromycin; central composite design; Chemometrics; chemometry; Computing time; Detectors; Flow rate; fluconazole; Formulations; Liquid chromatography; Methyl alcohol; RP-HPLC; secnidazole

This paper demonstrates a chemometric-assisted RP-HPLC method for the simultaneous determination of azithromycin, secnidazole, and fluconazole using water:methanol (63:37% v/v) as mobile phase at a flow rate of 1.2 mL · min −1 . The separation of the three drugs was achieved on an Xterra RP-18 colum...