Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag - 卢敏 龚兴国 于红 郦剑勇 (Journal of Zhejiang University. B. Science, 2005)
Keywords: Adenocarcinoma - immunology; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - isolation & purification; Cell Line; Cell Line, Tumor; Cloning, Molecular - methods; GFP; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; Humans; Hybridomas - metabolism; Immunoglobulin Fragments - biosynthesis; Immunoglobulin Fragments - genetics; Immunoglobulin Fragments - immunology; Immunoglobulin Fragments - isolation & purification; Lung Neoplasms - immunology; Plant & Animal Sciences and Biotechnology; Protein Engineering - methods; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - immunology; Recombinant Fusion Proteins - isolation & purification; RNA; 克隆技术; 基因工程; 绿色荧光蛋白质; 遗传表达; Protein fusion; ScFv (single chain variable fragment); Purification; GFP (green fluorescent protein) tag