Cell to cell transfer of the chromatin-packaged human β-globin gene cluster

• Published in: Nucleic acids research Vol. 38; no. 5; p. e33
• Main Authors: Suzuki, Nobutaka, Itou, Toshihide, Hasegawa, Yoshinori, Okazaki, Tsuneko, Ikeno, Masashi
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.03.2010
• Subjects: Animals; beta-Globins - genetics; beta-Globins - metabolism; Cell Differentiation; Cell Line; Chromatin - genetics; Chromosomes, Artificial, Human; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Erythrocytes - cytology; Erythrocytes - metabolism; Gene Expression Regulation; Gene Transfer Techniques; Humans; K562 Cells; Methods Online; Mice; Mice, Transgenic; Multigene Family

Cell type-specific gene expression is regulated by chromatin structure and the transcription factors provided by the cells. In the present study, we introduced genes packaged into chromatin into target cells using a human artificial chromosome (HAC) and analyzed regulation of gene expression. The hu...