Development of non-adherent cell-enclosing domes with enzymatically cross-linked hydrogel shell

• Published in: Biofabrication Vol. 15; no. 1; pp. 15002 - 15011
• Main Authors: Kazama, Ryotaro, Sato, Ryuta, Fujiwara, Hiroyuki, Qu, Yanfei, Nakahata, Masaki, Kojima, Masaru, Fujita, Satoshi, Sakai, Shinji
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 01.01.2023
• Subjects: alginate; Alginates - metabolism; Cell Dome; Horseradish Peroxidase; Humans; Hydrogels; K562 cell; microcapsule; microdome; Mitomycin; non-adherent cell; alginate; K562 cell; microcapsule; microdome; Cell Dome; non-adherent cell

Non-adherent cells, such as hematopoietic cells and lymphocytes, are important research subjects in medical and biological fields. Therefore, a system that enables the handling of non-adherent cells in solutions in the same manner as that of adhering cells during medium exchange, exposure to chemica...