Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus

• Published in: Nature (London) Vol. 287; no. 5781; p. 396
• Main Authors: Windass, J D, Worsey, M J, Pioli, E M, Pioli, D, Barth, P T, Atherton, K T, Dart, E C, Byrom, D, Powell, K, Senior, P J
• Format: Journal Article
• Language: English
• Published: England 02.10.1980
• Subjects: Adenosine Triphosphate - metabolism; Ammonia - metabolism; Bacterial Proteins - biosynthesis; DNA, Recombinant; Escherichia coli - enzymology; Genetic Engineering - methods; Glutamate Dehydrogenase - genetics; Glutamate Synthase - metabolism; Methanol - metabolism; Methylococcaceae - enzymology; Methylococcaceae - metabolism; Plasmids

The glutamate dehydrogenase gene of Escherichia coli has been cloned into broad host-range plasmids and can complement glutamate synthase mutants of Methylophilus methylotrophus. Assimilation of ammonia via glutamate dehydrogenase is more energy-efficient than via glutamate synthase, thus the recomb...