Isolation and amino acid sequence of a novel 6.8-kDa mitochondrial proteolipid from beef heart: Use of FAB-MS for molecular mass determination

We have isolated a 6.8 kDa proteolipid from an acidic chloroform/methanol extract of bovine cardiac muscle. The molecular mass of the polypeptide was measured by fast atom bombardment-mass spectrometry (FAB-MS) (m/z 6834.1). Its amino acid sequence was partly determined by direct sequencing and comp...

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Published inFEBS letters Vol. 260; no. 1; pp. 122 - 126
Main Authors Terzi, E., Boyot, P., Van Dorsselaer, A., Luu, B., Trifilieff, E.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 15.01.1990
Elsevier
Subjects
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Bovine heart
Cattle
Chromatography, High Pressure Liquid
Fast atom bombardment-mass spectrometry
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Hydrolysis
Immunoblotting
lipoproteins
Lipoproteins, myelin
Mass Spectrometry
Mitochondria
Mitochondria, Heart - analysis
Molecular Sequence Data
Molecular Weight
Peptide Fragments - isolation & purification
Peptides - isolation & purification
Proteins
Proteolipid
Proteolipids - isolation & purification
Solvents
Amino acid sequence
Bovine heart
Mitochondria
Fast atom bombardment-mass spectrometry
Proteolipid
Heart
Purification
Bovine
FAB mass spectrum
Vertebrata
Mammalia
Immunoblotting assay
Muscle
Artiodactyla
Mass spectrometry
Ungulata
Aminoacid sequence
Online AccessGet full text

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Abstract We have isolated a 6.8 kDa proteolipid from an acidic chloroform/methanol extract of bovine cardiac muscle. The molecular mass of the polypeptide was measured by fast atom bombardment-mass spectrometry (FAB-MS) (m/z 6834.1). Its amino acid sequence was partly determined by direct sequencing and completed by characterization of cyanogen bromide and tryptic fragments (sequencing, FAB-MS and amino acid analysis). The polypeptide consists of 60 amino acid residues. Polyclonal antibodies raised in rabbit allowed its localization by electroimmunoblotting in mitochondria.
AbstractList We have isolated a 6.8 kDa proteolipid from an acidic chloroform/methanol extract of bovine cardiac muscle. The molecular mass of the polypeptide was measured by fast atom bombardment-mass spectrometry (FAB-MS) (m/z 6834.1). Its amino acid sequence was partly determined by direct sequencing and completed by characterization of cyanogen bromide and tryptic fragments (sequencing, FAB-MS and amino acid analysis). The polypeptide consists of 60 amino acid residues. Polyclonal antibodies raised in rabbit allowed its localization by electroimmunoblotting in mitochondria.
We have isolated a 6.8 kDa proteolipid from an acidic chloroform/methanol extract of bovine cardiac muscle. The molecular mass of the polypeptide was measured by fast atom bombardment-mass spectrometry (FAB-MS) (m/z6834.1). Its amino acid sequence was partly determined by direct sequencing and completed by characterization of cyanogen bromide and tryptic fragments (sequencing, FAB-MS and amino acid analysis). The polypeptide consists of 60 amino acid residues. Polyclonal antibodies raised in rabbit allowed its localization by electroimmunoblotting in mitochondria.
Author Terzi, E.
Boyot, P.
Luu, B.
Trifilieff, E.
Van Dorsselaer, A.
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Cites_doi 10.1002/j.1460-2075.1986.tb04456.x
10.1016/0003-2697(88)90162-5
10.1016/0014-5793(86)80412-4
10.1016/0003-9861(89)90149-5
10.1016/0003-9861(83)90334-X
10.1073/pnas.76.9.4350
10.1016/S0076-6879(83)91043-1
10.1016/0076-6879(79)55006-X
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Issue 1
Keywords Amino acid sequence
Bovine heart
Mitochondria
Fast atom bombardment-mass spectrometry
Proteolipid
Heart
Purification
Bovine
FAB mass spectrum
Vertebrata
Mammalia
Immunoblotting assay
Muscle
Artiodactyla
Mass spectrometry
Ungulata
Aminoacid sequence
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References Boyot, Trifilieff, Van, Luu (BIB2) 1988; 173
Trifilieff, Luu, Nussbaum, Roussel, Espinosa, Sabatier, Van (BIB6) 1986; 198
Towbin, Staehelin, Gordon (BIB5) 1979; 76
Chang (BIB4) 1983; 91
Fearnley, Walker (BIB8) 1986; 5
Lees, Chao, Lin, Samiullah, Laursen (BIB3) 1983; 226
Mela, Seitz (BIB7) 1979; 55
Boyot, Luu, Jones, Trifilieff (BIB1) 1989; 269
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  year: 1989
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  publication-title: Arch. Biochem. Biophys.
  contributor:
    fullname: Trifilieff
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  start-page: 455
  year: 1983
  end-page: 466
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  publication-title: Method Enzymol.
  contributor:
    fullname: Chang
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  start-page: 2003
  year: 1986
  end-page: 2008
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  publication-title: Methods Enzymol.
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    fullname: Seitz
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  start-page: 75
  year: 1988
  end-page: 85
  ident: BIB2
  publication-title: Anal. Biochem.
  contributor:
    fullname: Luu
– volume: 198
  start-page: 235
  year: 1986
  end-page: 239
  ident: BIB6
  publication-title: FEBS Lett.
  contributor:
    fullname: Van
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  start-page: 4350
  year: 1979
  end-page: 4354
  ident: BIB5
  publication-title: Proc. Natl. Acad. Sci. USA
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    fullname: Gordon
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Snippet We have isolated a 6.8 kDa proteolipid from an acidic chloroform/methanol extract of bovine cardiac muscle. The molecular mass of the polypeptide was measured...
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SubjectTerms Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Bovine heart
Cattle
Chromatography, High Pressure Liquid
Fast atom bombardment-mass spectrometry
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Hydrolysis
Immunoblotting
lipoproteins
Lipoproteins, myelin
Mass Spectrometry
Mitochondria
Mitochondria, Heart - analysis
Molecular Sequence Data
Molecular Weight
Peptide Fragments - isolation & purification
Peptides - isolation & purification
Proteins
Proteolipid
Proteolipids - isolation & purification
Solvents
Title Isolation and amino acid sequence of a novel 6.8-kDa mitochondrial proteolipid from beef heart: Use of FAB-MS for molecular mass determination
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