An in vivo target mutagenesis system for multiple hosts

• Published in: Trends in biotechnology (Regular ed.) Vol. 43; no. 8; pp. 2049 - 2072
• Main Authors: Yin, Dong, Pang, Qingxiao, Yuan, Yingbo, Su, Tianyuan, Liu, Mengmeng, Wang, Qian, Hou, Jin, Qi, Qingsheng
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2025; Elsevier Limited
• Subjects: Adenosine; Bacteria; Biological evolution; Corynebacterium glutamicum - genetics; Directed Molecular Evolution - methods; DNA helicase; DNA polymerase; DNA primase; DNA-directed DNA polymerase; E coli; Enzymes; Escherichia coli - genetics; Evolution; Genes; Genomes; in vivo target mutagenesis; Internal Medicine; Metabolism; Microorganisms; multiple hosts; Mutagenesis; Mutation; Mutation rates; Plasmids; Plasmids - genetics; Primase; Proteins; Pseudomonas putida; Pseudomonas putida - genetics; synthetic biology; Yarrowia - genetics; in vivo target mutagenesis; synthetic biology; multiple hosts

In vivo targeted mutagenesis technology can enable the gene of interest to generate genetic diversity within the host cell without human intervention.We developed an in vivo targeted mutagenesis system applicable to multiple hosts, effectively addressing the key gap of in vivo mutagenesis tools for...