Experimental study of the formation of high-resistivity zones at the gel/buffer interface in CE

• Published in: Electrophoresis Vol. 28; no. 3; pp. 317 - 321
• Main Authors: Khozikov, Vyacheslav, Kosobokova, Olga, Citver, Gregory, Tyshko, George, Gavrilov, Dmitri N., Gudkov, George, Gorfinkel, Vera
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.02.2007; WILEY‐VCH Verlag
• Subjects: Buffers; Computer Simulation; Electric Conductivity; Electrophoresis, Capillary; High-resistivity zone; Joule heating; Sequence Analysis, DNA - instrumentation; Sequence Analysis, DNA - methods; Temperature

A novel, nondamaging method for experimental characterization of the formation and propagation of high‐resistivity zones in CE, based on the measurement of time‐dependent Joule heating on the outer capillary surface is proposed. The method detects propagation of resistive regions in capillaries in r...