Experimental study of the formation of high-resistivity zones at the gel/buffer interface in CE

A novel, nondamaging method for experimental characterization of the formation and propagation of high‐resistivity zones in CE, based on the measurement of time‐dependent Joule heating on the outer capillary surface is proposed. The method detects propagation of resistive regions in capillaries in r...

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Published inElectrophoresis Vol. 28; no. 3; pp. 317 - 321
Main Authors Khozikov, Vyacheslav, Kosobokova, Olga, Citver, Gregory, Tyshko, George, Gavrilov, Dmitri N., Gudkov, George, Gorfinkel, Vera
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.02.2007
WILEY‐VCH Verlag
Subjects
Buffers
Computer Simulation
Electric Conductivity
Electrophoresis, Capillary
High-resistivity zone
Joule heating
Sequence Analysis, DNA - instrumentation
Sequence Analysis, DNA - methods
Temperature
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Abstract A novel, nondamaging method for experimental characterization of the formation and propagation of high‐resistivity zones in CE, based on the measurement of time‐dependent Joule heating on the outer capillary surface is proposed. The method detects propagation of resistive regions in capillaries in real time and allows the estimation of their velocity and resistance. The presented experimental data are in agreement with the results of the computer simulation as well as with previous data on the subject. The proposed method is useful for the development of new polymers as well as for the refinement and optimization of new CE protocols.
AbstractList A novel, nondamaging method for experimental characterization of the formation and propagation of high-resistivity zones in CE, based on the measurement of time-dependent Joule heating on the outer capillary surface is proposed. The method detects propagation of resistive regions in capillaries in real time and allows the estimation of their velocity and resistance. The presented experimental data are in agreement with the results of the computer simulation as well as with previous data on the subject. The proposed method is useful for the development of new polymers as well as for the refinement and optimization of new CE protocols.
A novel, nondamaging method for experimental characterization of the formation and propagation of high-resistivity zones in CE, based on the measurement of time-dependent Joule heating on the outer capillary surface is proposed. The method detects propagation of resistive regions in capillaries in real time and allows the estimation of their velocity and resistance. The presented experimental data are in agreement with the results of the computer simulation as well as with previous data on the subject. The proposed method is useful for the development of new polymers as well as for the refinement and optimization of new CE protocols.A novel, nondamaging method for experimental characterization of the formation and propagation of high-resistivity zones in CE, based on the measurement of time-dependent Joule heating on the outer capillary surface is proposed. The method detects propagation of resistive regions in capillaries in real time and allows the estimation of their velocity and resistance. The presented experimental data are in agreement with the results of the computer simulation as well as with previous data on the subject. The proposed method is useful for the development of new polymers as well as for the refinement and optimization of new CE protocols.
Author Gudkov, George
Gorfinkel, Vera
Kosobokova, Olga
Gavrilov, Dmitri N.
Tyshko, George
Khozikov, Vyacheslav
Citver, Gregory
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Snippet A novel, nondamaging method for experimental characterization of the formation and propagation of high‐resistivity zones in CE, based on the measurement of...
A novel, nondamaging method for experimental characterization of the formation and propagation of high-resistivity zones in CE, based on the measurement of...
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SubjectTerms Buffers
Computer Simulation
Electric Conductivity
Electrophoresis, Capillary
High-resistivity zone
Joule heating
Sequence Analysis, DNA - instrumentation
Sequence Analysis, DNA - methods
Temperature
Title Experimental study of the formation of high-resistivity zones at the gel/buffer interface in CE
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